OTC 抗体 (N-Term)
Quick Overview for OTC 抗体 (N-Term) (ABIN5518860)
抗原
See all OTC 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 33-70, N-Term
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原理
- Anti-Ornithine Carbamoyltransferase/OTC Antibody Picoband®
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序列
- NKVQLKGRDL LTLKNFTGEE IKYMLWLSAD LKFRIKQK
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-Ornithine Carbamoyltransferase/OTC Antibody Picoband® (ABIN5518860). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human OTC, different from the related mouse and rat sequences by three amino acids.
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亚型
- IgG
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应用备注
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Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. Langley DB, Templeton MD, Fields BA, Mitchell RE, Collyer CA (June 2000). "Mechanism of inactivation of ornithine transcarbamoylase by Ndelta -(N'-Sulfodiaminophosphinyl)-L-ornithine, a true transition state analogue? Crystal structure and implications for catalytic mechanism". J. Biol. Chem. 275 (26): 20012-9. 2. Nagamine T, Saito S, Kaneko M, Sekiguchi T, Sugimoto H, Takehara K, Takagi H (June 1995). "Effect of biotin on ammonia intoxication in rats and mice". J. Gastroenterol. 30 (3): 351-5. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- OTC (Ornithine Carbamoyltransferase (OTC))
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别名
- OTC
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背景
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Synonyms: Ornithine carbamoyltransferase, mitochondrial,2.1.3.3,Ornithine transcarbamylase,OTCase,OTC,
Tissue Specificity: Mainly expressed in liver and intestinal mucosa.
Background: Ornithine transcarbamylase (OTC) (also called ornithine carbamoyltransferase) is an enzyme that catalyzes the reaction between carbamoyl phosphate (CP) and ornithine (Orn) to form citrulline (Cit) and phosphate (Pi). This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may also play a role in that disease.
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分子量
- 40 kDa
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基因ID
- 5009
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UniProt
- P00480
抗原
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