Choline Acetyltransferase 抗体 (AA 446-652)
(7 references)Quick Overview for Choline Acetyltransferase 抗体 (AA 446-652) (ABIN5518818)
抗原
See all Choline Acetyltransferase (CHAT) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 446-652
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原理
- Anti-Choline Acetyltransferase/CHAT Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Choline Acetyltransferase/CHAT Antibody Picoband® (ABIN5518818). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human CHAT recombinant protein (Position: D446-R652). Human CHAT shares 88.4% and 87% amino acid (aa) sequence identity with mouse and rat CHAT, respectively.
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亚型
- IgG
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anti-Choline O-Acetyltransferase (CHAT) (AA 517-732) antibody
CHAT 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) (AA 331-630) antibodyCHAT 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) (AA 98-128) antibodyCHAT 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) (AA 101-200) antibodyCHAT 适用: 人, 小鼠, 大鼠, 犬 ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) (AA 354-592) antibodyCHAT 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) (C-Term) antibodyVerified CHAT 适用: 小鼠, 大鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) antibodyCHAT 适用: 人 WB, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) antibodyMicroarray verified CHAT 适用: 人 IHC 宿主: 小鼠 Monoclonal MAB872084 unconjugated
anti-Choline O-Acetyltransferase (CHAT) (N-Term) antibody (HRP)CHAT 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal HRP
anti-Choline O-Acetyltransferase (CHAT) (N-Term) antibody (FITC)CHAT 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal FITC
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应用备注
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Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat, Human
Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. Berman R, Wilson IB, Nachmansohn D (September-October 1953). "Choline acetylase specificity in relation to biological function.". Biochimica et Biophysica Acta. 12 (1-2): 315-24. 2. Strauss WL, Kemper RR, Jayakar P, Kong CF, Hersh LB, Hilt DC, Rabin M (February 1991). "Human choline acetyltransferase gene maps to region 10q11-q22.2 by in situ hybridization". Genomics. 9 (2): 396-8. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "Acetylcholine acts through M3 muscarinic receptor to activate the EGFR signaling and promotes gastric cancer cell proliferation." in: Scientific reports, Vol. 7, pp. 40802, (2018) (PubMed).
: "Pharmacologic activation of cholinergic alpha7 nicotinic receptors mitigates depressive-like behavior in a mouse model of chronic stress." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 234, (2018) (PubMed).
: "Autonomic remodeling may be responsible for decreased incidence of aortic dissection in STZ-induced diabetic rats via down-regulation of matrix metalloprotease 2." in: BMC cardiovascular disorders, Vol. 16, Issue 1, pp. 200, (2017) (PubMed).
: "Overexpression of NTRK1 Promotes Differentiation of Neural Stem Cells into Cholinergic Neurons." in: BioMed research international, Vol. 2015, pp. 857202, (2016) (PubMed).
: "Wnt3a expression during the differentiation of adipose-derived stem cells into cholinergic neurons." in: Neural regeneration research, Vol. 7, Issue 19, pp. 1463-8, (2015) (PubMed).
: "Thalidomide influences growth and vasculogenic mimicry channel formation in melanoma." in: Journal of experimental & clinical cancer research : CR, Vol. 27, pp. 60, (2009) (PubMed).
: "Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer." in: Journal of experimental & clinical cancer research : CR, Vol. 27, pp. 62, (2009) (PubMed).
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: "Acetylcholine acts through M3 muscarinic receptor to activate the EGFR signaling and promotes gastric cancer cell proliferation." in: Scientific reports, Vol. 7, pp. 40802, (2018) (PubMed).
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- Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))
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别名
- CHAT
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背景
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Synonyms: Choline O-acetyltransferase,CHOACTase,ChAT,Choline acetylase,2.3.1.6,CHAT,
Background: Choline acetyltransferase (commonly abbreviated as ChAT, but sometimes CAT) is a transferase enzyme responsible for the synthesis of the neurotransmitter acetylcholine. In humans, the choline acetyltransferase enzyme is encoded by the CHAT gene. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.
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分子量
- 83 kDa
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基因ID
- 1103
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UniProt
- P28329
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途径
- Skeletal Muscle Fiber Development
抗原
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