PPL 抗体 (C-Term)
Quick Overview for PPL 抗体 (C-Term) (ABIN5518787)
抗原
See all PPL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1664-1701, C-Term
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原理
- Anti-Periplakin/PPL Antibody Picoband®
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序列
- DTGRELSPEE AHRAGLIDWN MFVKLRSQEC DWEEISVK
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Periplakin/PPL Antibody Picoband® (ABIN5518787). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human Periplakin, different from the related mouse sequence by one amino acid.
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亚型
- IgG
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anti-Periplakin (PPL) (AA 1664-1701) antibody
PPL 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Periplakin (PPL) (AA 1-130) antibodyPPL 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Periplakin (PPL) (AA 1501-1754) antibodyPPL 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Periplakin (PPL) antibodyPPL 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Periplakin (PPL) antibodyPPL 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Periplakin (PPL) (AA 1044-1093) antibodyPPL 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Periplakin (PPL) (AA 1-130) antibodyPPL 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Periplakin (PPL) (AA 1439-1716) antibodyPPL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Periplakin (PPL) (AA 1301-1330), (C-Term) antibodyPPL 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. "Entrez Gene: PPL periplakin". 2. Aho S, McLean WH, Li K, Uitto J (Jun 1998). "cDNA cloning, mRNA expression, and chromosomal mapping of human and mouse periplakin genes". Genomics. 48 (2): 242-7. 3. Kazerounian, Shideh, Uitto Jouni, Aho Sirpa (Oct 2002). "Unique role for the periplakin tail in intermediate filament association: specific binding to keratin 8 and vimentin". Exp. Dermatol. Denmark. 11(5): 428-38. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- PPL (Periplakin (PPL))
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别名
- PPL
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背景
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Synonyms: Periplakin,190 kDa paraneoplastic pemphigus antigen,195 kDa cornified envelope precursor protein,PPL,KIAA0568,
Tissue Specificity: Expressed in stratified squamous epithelia and in some other epithelia. .
Background: Periplakin is a protein that in humans is encoded by the PPL gene. The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling.
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分子量
- 202 kDa
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基因ID
- 5493
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UniProt
- O60437
抗原
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