ALOX12 抗体 (C-Term)
Quick Overview for ALOX12 抗体 (C-Term) (ABIN5518671)
抗原
See all ALOX12 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 609-628, C-Term
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原理
- Anti-12 Lipoxygenase/ALOX12 UBB Antibody Picoband®
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序列
- HHKEKYFSGP KPKAVLNQFR
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-12 Lipoxygenase/ALOX12 UBB Antibody (ABIN5518671). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human ALOX12, different from the related rat sequence by four amino acids, and from the related mouse sequence by five amino acids.
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亚型
- IgG
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anti-Arachidonate 12-Lipoxygenase (ALOX12) (AA 352-656) antibody
ALOX12 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Arachidonate 12-Lipoxygenase (ALOX12) (AA 508-658) antibodyALOX12 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Arachidonate 12-Lipoxygenase (ALOX12) antibodyALOX12 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Arachidonate 12-Lipoxygenase (ALOX12) (AA 618-650), (C-Term) antibodyALOX12 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB17150 unconjugated
anti-Arachidonate 12-Lipoxygenase (ALOX12) (AA 564-663) antibodyALOX12 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Arachidonate 12-Lipoxygenase (ALOX12) (AA 564-663) antibodyALOX12 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D10 unconjugated
anti-Arachidonate 12-Lipoxygenase (ALOX12) (AA 1-663) antibodyALOX12 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Arachidonate 12-Lipoxygenase (ALOX12) antibodyALOX12 适用: 人 WB, IHC, FACS, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Arachidonate 12-Lipoxygenase (ALOX12) (AA 618-650) antibodyALOX12 适用: 人 WB, IHC, IF, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Arachidonate 12-Lipoxygenase (ALOX12) (AA 1-663) antibodyALOX12 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
1. Funk, C. D., Furci, L., FitzGerald, G. A.Molecular cloning, primary structure, and expression of the human platelet/erythroleukemia cell 12-lipoxygenase.Proc. Nat. Acad. Sci. 87: 5638-5642, 1990. 2. Natarajan, R., Esworthy, R., Bai, W., Gu, J.-L., Wilczynski, S., Nadler, J.Increased 12-lipoxygenase expression in breast cancer tissues and cells. Regulation by epidermal growth factor.J. Clin. Endocr. Metab. 82: 1790-1798, 1997. 3. Yoshimoto, T., Arakawa, T., Hada, T., Yamamoto, S., Takahashi, E.Structure and chromosomal localization of human arachidonate 12-lipoxygenase gene.J. Biol. Chem. 267: 24805-24809, 1992. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Sodium azide, Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ALOX12 (Arachidonate 12-Lipoxygenase (ALOX12))
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别名
- ALOX12
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背景
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Synonyms: Polyubiquitin-B,Ubiquitin,UBB,
Tissue Specificity: Expressed in vascular smooth muscle cells.
Background: ALOX12, Arachidonate 12-lipoxygenase, is an enzyme that in humans is encoded by the ALOX12 gene. By fluorescence in situ hybridization, the ALOX12 gene is located in band 17p13.1. The gene consists of 14 exons with 13 introns and spans approximately 15 kb of DNAArachidonate 12-lipoxygenase introduces a molecular oxygen into the C-12 position of arachidonic acid to produce 12 (S)-hydroperoxy-5,8,10,14-eicosatetraenoic acid. The major pathway of arachidonic acid metabolism in human platelets proceeds via a 12-lipoxygenase enzyme. Expression of the LOG12 gene was detected in human erythroleukemia cells, platelets, and human umbilical vein endothelial cells by reverse transcription-PCR analysis.
Sequence Similarities: Belongs to the lipoxygenase family.
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分子量
- 75 kDa
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途径
- Positive Regulation of Endopeptidase Activity
抗原
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