ALPL 抗体 (N-Term)
(20 references)Quick Overview for ALPL 抗体 (N-Term) (ABIN5518639)
抗原
See all ALPL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 18-32, N-Term
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原理
- Anti-Alkaline Phosphatase/ALPL Antibody Picoband®
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序列
- FVPEKEKDPS YWRQQ
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Alkaline Phosphatase/ALPL Antibody (ABIN5518639). Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of rat Alkaline Phosphatase, different from the related mouse sequence by one amino acid.
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亚型
- IgG
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anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 18-335) antibody
ALPL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) antibodyALPL 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 2F4 unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) antibodyALPL 适用: 人, Cow IHC, StM 宿主: 小鼠 Monoclonal ALPL-597 unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 21-35), (N-Term) antibodyALPL 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 217-246) antibodyALPL 适用: 人, 小鼠 WB, ELISA, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 91-200) antibodyALPL 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 4H1 unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (Internal Region) antibodyALPL 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 20-270) antibodyALPL 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) antibodyALPL 适用: 人 WB, IHC (p), FACS, EIA 宿主: 小鼠 Monoclonal 2F4 unconjugated
anti-Alkaline Phosphatase, Liver/bone/kidney (ALPL) (AA 52-150) antibodyALPL 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Rat, Mouse
1. Weiss MJ, Henthorn PS, Lafferty MA, Slaughter C, Raducha M, Harris H (Oct 1986). "Isolation and characterization of a cDNA encoding a human liver/bone/kidney-type alkaline phosphatase". Proc Natl Acad Sci U S A 83 (19): 7182-6. 2. Swallow DM, Povey S, Parkar M, Andrews PW, Harris H, Pym B, Goodfellow P (May 1988). "Mapping of the gene coding for the human liver/bone/kidney isozyme of alkaline phosphatase to chromosome 1". Ann Hum Genet 50 (Pt 3): 229-35. 3. "Entrez Gene: ALPL alkaline phosphatase, liver/bone/kidney" 4. Fedde, K. N., Whyte, M. P. Alkaline phosphatase (tissue-nonspecific isoenzyme) is a phosphoethanolamine and pyridoxal-5-prime-phosphate ectophosphatase: normal and hypophosphatasia fibroblast study. Am. J. Hum. Genet. 47: 767-775, 1990. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Sodium azide, Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "Cartilage oligomeric matrix protein gene multilayers inhibit osteogenic differentiation and promote chondrogenic differentiation of mesenchymal stem cells." in: International journal of molecular sciences, Vol. 15, Issue 11, pp. 20117-33, (2014) (PubMed).
: "Amelioration of compound 4,4'-diphenylmethane-bis(methyl)carbamate on high mobility group box1-mediated inflammation and oxidant stress responses in human umbilical vein endothelial cells via ..." in: International immunopharmacology, Vol. 15, Issue 2, pp. 206-16, (2013) (PubMed).
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: "Rho kinase inhibitor Y-27632 and Accutase dramatically increase mouse embryonic stem cell derivation." in: In vitro cellular & developmental biology. Animal, Vol. 48, Issue 1, pp. 30-6, (2012) (PubMed).
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: "Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts." in: Molecular vision, Vol. 17, pp. 247-56, (2011) (PubMed).
: "Differentiation of human mesenchymal stem cells: the potential mechanism for estrogen-induced preferential osteoblast versus adipocyte differentiation." in: The American journal of the medical sciences, Vol. 341, Issue 6, pp. 460-8, (2011) (PubMed).
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: "Significance and relationship between DJ-1 gene and surviving gene expression in laryngeal carcinoma." in: European journal of histochemistry : EJH, Vol. 55, Issue 1, pp. e9, (2011) (PubMed).
: "Derivation and characteristics of pluripotent embryonic germ cells in duck." in: Poultry science, Vol. 89, Issue 2, pp. 312-7, (2010) (PubMed).
: "Study on environmental estrogen pollution in Yangtze River (Nanjing section) by an in vivo bioassay." in: Bulletin of environmental contamination and toxicology, Vol. 84, Issue 4, pp. 406-12, (2010) (PubMed).
: "Assessment of in vivo estrogenic response and the identification of environmental estrogens in the Yangtze River (Nanjing section)." in: Chemosphere, Vol. 80, Issue 9, pp. 982-90, (2010) (PubMed).
: "Mimotope vaccination for epitope-specific induction of anti-CD20 antibodies." in: Cellular immunology, Vol. 239, Issue 2, pp. 136-43, (2006) (PubMed).
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: "Decreasing the ratio of matriptase/HAI‑1 by downregulation of matriptase as a potential adjuvant therapy in ovarian cancer." in: Molecular medicine reports, Vol. 14, Issue 2, pp. 1465-74, (2017) (PubMed).
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- ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))
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别名
- ALPL
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背景
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Synonyms: Alkaline phosphatase, tissue-nonspecific isozyme,AP-TNAP,TNSALP,3.1.3.1,Alkaline phosphatase liver/bone/kidney isozyme,Alpl,
Tissue Specificity: Widely expressed, with highest expression in brain. .
Background: Alkaline phosphatase, tissue-nonspecific isozyme is an enzyme that in humans is encoded by the ALPL gene. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. Liver/bone/kidney alkaline phosphatase, also known as tissue-nonspecific alkaline phosphatase, acts physiologically as a lipid-anchored phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP) ectophosphatase Mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked ly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects.
Sequence Similarities: Contains 12 fibronectin type-I domains.
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分子量
- 57 kDa
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UniProt
- P08289
抗原
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