beta Amyloid 抗体 (C-Term)
(4 references)
(2 validations)Quick Overview for beta Amyloid 抗体 (C-Term) (ABIN5508784)
抗原
See all beta Amyloid (Abeta) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- C-Term
-
特异性
- A-beta 1-40, A-beta 1-42, A-beta 1-43 and APP
-
交叉反应 （详细）
- Human, mouse, rat (other species not tested)
-
纯化方法
- Protein G affinity purification
-
免疫原
- Synthetic peptide derived from Human Abeta 1-42 protein
-
亚型
- IgG1
-
-
anti-Amyloid beta (Abeta) (AA 711-760) antibody
Abeta 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (Abeta) antibodyAbeta 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (Abeta) (Ser15) antibodyAbeta 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (Abeta) antibodyAbeta 适用: 人 WB, ELISA, IHC, IP, IEM 宿主: 兔 Chimeric 6E10 unconjugated Recombinant Antibody
anti-Amyloid beta (Abeta) (pThr743) antibodyAbeta 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (Abeta) antibodyAbeta 适用: 人 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal AB9 unconjugated
anti-Amyloid beta (Abeta) (C-Term), (pSer743) antibodyAbeta 适用: 人, 小鼠, 大鼠, Pig, 犬, 小鸡, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- ELISA: 1:40,000, WB: 1:4,000, IF: 1:2,000
-
限制
- 仅限研究用
-
-
- by
- Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
- No.
- #104425
- 日期
- 2022.05.12
- 抗原
- beta Amyloid
- Lot Number
- 190522A
- Method validated
- Immunohistochemistry
- Positive Control
Adult (24 months) mouse brain
3-month-old Taconic mouse Model 1349 brain fixed in 4% paraformaldehyde
- Negative Control
We incubated slices overnight with the blocking solution only and then processed them with the secondary antibody.
- Notes
The beta amyloid antibody (Amyloid beta) (C-Term) ABIN5508784 works in IHC-P, especially at higher concentrations (1:200 and 1:500), and without the use of any antigen retrieval treatment.
- Primary Antibody
- ABIN5508784
- Secondary Antibody
- goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418)
- Full Protocol
- Perfuse mice with paraformaldehyde 4% in 0.1 M phosphate buffer pH 7.4 and post-fix in the same fixative for an additional 2 h at RT.
- Wash, dehydrate, and embed samples in paraffin wax.
- Wash several times with 0.01 M PBS.
- Cut intestines and brain with a microtome into 6µm sections and mount on glass slides.
- After paraffin removal, incubate sections for 1 h at RT in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) to block non-specific binding sites.
- Incubate sections with primary anti-beta amyloid (C-Term) (antibodies Online, ABIN5508784, lot 190522A) diluted 1:200, 1:500, 1:1000, and 1:2000 in PBS-BSA-PLL ON at RT.
- Wash sections 3x 5 min with 0.01 M PBS.
- Incubate sections with secondary goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418) diluted 1:500 in 0.1 M PBS for 1 h at RT.
- Wash sections 3x 5 min with 0.01M PBS.
- Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
- Acquire images with a fluorescence microscope and appropriate filter settings for AF488, e.g. Leica DM 6000B fluorescence microscope equipped with a digital camera at 40x magnification.
- Experimental Notes
Antigen retrieval treatment was also tested. In this case, sections were processed for microwave antigen retrieval for 10 min(95-100 °C) in 10 mM sodium citrate buffer (pH 6.0). After 20 min of spontaneous cooling, sections were washed twice for 5 min with distilled water and for 5 min with PBS.
生效 #104425 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Staining of beta-amyloid positive cells in the adult mouse brain using ABIN5508784 at 40x magnification.]()
Staining of beta-amyloid positive cells in the adult mouse brain using ABIN5508784 at 40x magnification.
Full Methods - by
- Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
- No.
- #104493
- 日期
- 2023.03.15
- 抗原
- Abeta
- Lot Number
- 190522A
- Method validated
- Immunohistochemistry
- Positive Control
3-month-old Taconic mouse Model 1349 brain fixed in 4% paraformaldehyde
- Negative Control
One control slice for each experimental procedure processed omitting the primary antibody; overnight incubation in diluent solution only.
- Notes
The beta Amyloid antibody (Amyloid beta) (C-Term) ABIN5508784 works in IHC-P, especially at higher concentrations (1:200 and 1:500). Tyramide amplification strongly enhances the positive signal.
- Primary Antibody
- ABIN5508784
- Secondary Antibody
- poly-HRP conjugated goat anti-rabbit antibody
- Full Protocol
- Perfuse mice with paraformaldehyde 4% in 0.1 M phosphate buffer pH 7.4 and post-fix in the same fixative for an additional 2 h at RT.
- Wash, dehydrate, and embed samples in paraffin wax.
- Wash several times with 0.01 M PBS.
- Cut intestines and brain with a microtome into 6 µm sections and mount on glass slides.
- After paraffin removal, incubate sections for 1 h at RT in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) to block non-specific binding sites.
- Incubate sections with primary anti-beta amyloid (N-Term) (antibodies-online, ABIN5508784, lot 190522A) diluted 1:200, 1:500, 1:1000, and 1:2000 in 0.1 M PBS-BSA-PLL ON at RT.
- Wash sections 3x 5 min with 0.01 M PBS.
- Incubate sections with poly-HRP conjugated goat anti-rabbit antibody for 1 h at RT.
- Wash sections 3x 5 min with 0.01 M PBS.
- Incubate sections Tyramide working solution (for 5 sections: 100X Tyramide stock solution 5 μL, 100X H2O2 solution 5 μL, and 1X Reaction buffer 500 μL; Thermo Fisher Scientific, B40915, lot 2086865) for 10 min.
- Stop the reaction with the Reaction Stop Reagent working solution.
- Wash sections 3x 5 min with 0.01M PBS.
- Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
- Acquire images with a fluorescence microscope and appropriate filter settings for AF488, e.g. Leica DM 6000B fluorescence microscope equipped with a digital camera at 40x magnification.
- Experimental Notes
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- PBS, pH 7.4
-
储存液
- Without preservative
-
注意事项
- Avoid freezing/thaw cycles.
-
储存条件
- -20 °C
-
储存方法
- Store at -20°C to -80°C.
-
-
-
: "ImmunoPEGliposome-mediated reduction of blood and brain amyloid levels in a mouse model of Alzheimer's disease is restricted to aged animals." in: Biomaterials, Vol. 112, pp. 141-152, (2016) (PubMed).
: "Anti-Aβ-MAb and dually decorated nanoliposomes: effect of Aβ1-42 peptides on interaction with hCMEC/D3 cells." in: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V, Vol. 81, Issue 1, pp. 49-56, (2012) (PubMed).
: "Versatile and efficient targeting using a single nanoparticulate platform: application to cancer and Alzheimer's disease." in: ACS nano, Vol. 6, Issue 7, pp. 5866-79, (2012) (PubMed).
: "The binding affinity of anti-Aβ1-42 MAb-decorated nanoliposomes to Aβ1-42 peptides in vitro and to amyloid deposits in post-mortem tissue." in: Biomaterials, Vol. 32, Issue 23, pp. 5489-97, (2011) (PubMed).
-
: "ImmunoPEGliposome-mediated reduction of blood and brain amyloid levels in a mouse model of Alzheimer's disease is restricted to aged animals." in: Biomaterials, Vol. 112, pp. 141-152, (2016) (PubMed).
-
- beta Amyloid (Abeta) (Amyloid beta (Abeta))
-
别名
- Amyloid beta
-
背景
- Amyloid beta (Aβ or Abeta) is a peptide of 36 - 43 amino acids derived from the cleavage of APP (Amyloid Precursor Protein) by β- and γ-secretases. It is involved in Alzheimer´s disease, among other severe neurological disorders. It has been proved that the Aβ could be a crucial target for these important diseases treatment.
-
途径
- Inflammasome
抗原
-
Staining of beta-amyloid positive neurons in the hippocampus and cerebral cortex of a 3-month-old transgenic mouse using ABIN5508784. Tyramide amplification. No counterstain.
