(2 references)-
- 抗原 See all SMT1 products
- SMT1 (Cycloartenol-C-24-Methyltransferase (SMT1))
- 适用
- Arabidopsis thaliana
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- Immunolocalization (IL), Western Blotting (WB)
- 交叉反应 （详细）
- Not reactive in: Hordeum vulgare, Triticum aestivum, Withania somnifera
- 预测反应
- Amborella trichopoda, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Eutrema salsugineum, Glycine max, Glycine soja, Gossypium mexicanum, Medicago truncatula, Populus trichopocarpa, Prunus persica, Ricinus communis, Theobroma cacao, Vitis vinifera,
- 产品特性
- Expected / apparent Molecular Weight of the Antigene: 38 kDa
- 纯化方法
- affinity purified
- 免疫原
- KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SMT1 Q9LM02, At5g13710
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- 应用备注
- 1: 500 - 1:1 000 with ECL Advance or + (WB), 1: 50 - 1: 100 (IL)
- 说明
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SMT1 antibody characterization in Western Blot and immunofluorescence labeling: Boutté Y et al. (2010). Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis. EMBO J. 29, 5465-5458.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 200 μL of sterile water.
- 注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - 储存条件
- -20 °C
- 储存方法
- store lyophilized/reconstituted at -20C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).
: "Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis." in: The EMBO journal, Vol. 29, Issue 3, pp. 546-58, (2010) (PubMed).
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: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).
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- 抗原
- SMT1 (Cycloartenol-C-24-Methyltransferase (SMT1))
- 别名
- Sterol methyltransferase 1 (SMT1) (SMT1 产品)
- 别名
- CEPHALOPOD antibody, CPH antibody, MSH12.18 antibody, MSH12_18 antibody, SAM:CYCLOARTENOL-C24-METHYLTRANSFERASE antibody, sterol methyltransferase 1 antibody, sterol methyltransferase 1 antibody, SMT1 antibody
- 背景
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AGI Code: At5g13710
SMT1 (EC=2.1.1.41) is an enzyme involved in steroid biosynthesis localized specific to the Endoplasmic Reticulum (ER), Boutte et al., 2009 . It is catalyzing the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol. Alternative names: Cycloartenol-C-24-methyltransferase, 24-sterol C-methyltransferase 1, Protein STEROL METHYLTRANSFERASE 1, Protein CEPHALOPOD - 分子量
- 38 kDa
- UniProt
- Q9LM02
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