SMT1 抗体
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北京 101111
Quick Overview for SMT1 抗体 (ABIN5326722)
抗原
适用
宿主
克隆类型
应用范围
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原理
- Anti-SMT1 | Sterol methyltransferase 1
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交叉反应 （详细）
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Arabidopsis thaliana
Not reactive in: Hordeum vulgare, Triticum aestivum, Solanum lycopersicum, Withania somnifera -
预测反应
- Amborella trichopoda,Brassica napus,Brassica rapa,Capsella rubella,Citrus clementina,Eutrema salsugineum,Glycine max,Glycine soja,Gossypium mexicanum,Medicago truncatula,Populus trichopocarpa,Prunus persica,Ricinus communis,Theobroma cacao,Vitis vinifera
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纯化方法
- Immunogen affinity purified serum
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免疫原
- KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SMT1 UniProt: Q9LM02
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应用备注
- 1 : 50-1 : 100 (IL), 1 : 500-1 : 1000 (WB)
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说明
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SMT1 antibody characterization in Western Blot and immunofluorescence labeling: Boutté Y et al. (2010). Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis. EMBO J. 29, 5465-5458.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 μL of sterile water
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).
: "Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis." in: The EMBO journal, Vol. 29, Issue 3, pp. 546-58, (2010) (PubMed).
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: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).
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- SMT1 (Cycloartenol-C-24-Methyltransferase (SMT1))
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别名
- Sterol methyltransferase 1 (SMT1)
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背景
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Background: SMT1 (EC=2.1.1.41) is an enzyme involved in steroid biosynthesis localized specific to the Endoplasmic Reticulum (ER), Boutte et al., 2009 . It is catalyzing the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol. The protein is highly expressed in vascular tissue, mature leaves and in regions undergoing cellular expansion. Alternative names: Cycloartenol-C-24-methyltransferase, 24-sterol C-methyltransferase 1, Protein STEROL METHYLTRANSFERASE 1, Protein CEPHALOPOD
Additional information: SMT1 is an integral membrane protein of ER (Boutte et al., 2009) while BiP is a membrane associated protein.Endogenous SMT1 is expressed at very low levels in root epidermis and root cap as compare to cortex and endodermis and therefore this can contribute to SMT1 detection problems.
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分子量
- 38 kDa
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基因ID
- 831216
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UniProt
- Q9LM02
抗原
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