STON1 抗体 (AA 529-620)
(1 validation)-
- 抗原 See all STON1 抗体
- STON1 (Stonin 1 (STON1))
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抗原表位
- AA 529-620
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This STON1 antibody is un-conjugated
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应用范围
- ELISA, Immunofluorescence (IF)
- 原理
- Mouse monoclonal antibody raised against a partial recombinant SBLF.
- 序列
- SLKSVVVVQG AYVELQAFVN MASLAQRSSY AGSLRSCDNI RIHFPVPSQW IKALWTMNLQ RQKSLKAKMN RRACLGSLQE LESEPVIQVT VG
- 交叉反应
- 人
- 产品特性
- Antibody Reactive Against Recombinant Protein.
- 免疫原
- SBLF (NP_006864, 529 a.a. ~ 620 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- 克隆位点
- 1F3
- 亚型
- IgG2a
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anti-Stonin 1 (STON1) (C-Term) antibody
STON1 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Stonin 1 (STON1) (C-Term) antibodySTON1 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Stonin 1 (STON1) (AA 660-740) antibodySTON1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Stonin 1 (STON1) (C-Term) antibodySTON1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Stonin 1 (STON1) (AA 1-735) antibodySTON1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Stonin 1 (STON1) (AA 686-735) antibodySTON1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Stonin 1 (STON1) (C-Term) antibodySTON1 适用: 人, 犬, 小鼠, Cow, 豚鼠, 马, 兔, 大鼠, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Stonin 1 (STON1) antibodySTON1 适用: 人, 犬, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- by
- Lab of Prof. Dr. S.A. Kushner, Department of Psychiatry, Erasmus University Medical Center
- No.
- #101553
- 日期
- 2018.01.12
- 抗原
- STON1
- Lot Number
- 08242-1F3
- Method validated
- Immunofluorescence
- Positive Control
- U-373 cell line (human glioblastoma astrocytoma)
- Negative Control
- secondary only
- Notes
The STON1 antibody ABIN524426 specifically labels the targeted antigen in sample in IF.
- Primary Antibody
- ABIN524426
- Secondary Antibody
- donkey anti-mouse A647-conjugated antibody (Sanbio, 715-605-150)
- Full Protocol
- Grow U-373 cells (human glioblastoma astrocytoma) in DMEM, high glucose, glutamax (Gibco, product number: 31966-021, lot number: 1894785 supplemented with Fetal Bovine Serum (Gibco, 10082-147, lot 1907451) and penicillin streptomycin (Gibco, 15140-122, lot 1910857), at 37°C and 5% CO2 in 2ml on a coverslips.
- Transfect cells with aCSPG4-GFP expression plasmid using X-tremeGENE HP (Sigma) accoding to the manufacturer’s recommendations.
- Fix cells on coverslips in 4% PFA for 20min at RT.
- Wash cells 3x for 5min with PBS.
- Wash coverslips for 5min with staining solution (50mM TrisHCl pH7.4, 154mM NaCl, 0.25% w/v gelatine, 5% v/v triton x-100).
- Incubate cells with primary mouse anti-human STON1 antibodylavivirus (antibodies-online, ABIN524426, lot 08242-1F3) diluted 1:100 in staining solution ON at 4°C.
- Wash cells 3x for 5min with PBS.
- Incubate cells with secondary donkey anti-mouse A647-conjugated antibody (Sanbio, 715-605-150) diluted 1:200 in staining solution for 1.5h at RT.
- Wash cells 2x for 5min with PBS.
- DAPI counterstain.
- Mount coverslips on glass slides in Mowiol 4-88 antifade reagent (Sigma, 81381, lot BCBL1789V).
- Image acquisition with LSM 700, 63x magnification, 1024x1024 resolution.
- Experimental Notes
ABIN524426 was used in cells overexpressing CSPG4 fused to GFP. Stonin is expected to be abundantly expressed in the cells.
No signal was detected in the secondary antibody only control (not shown).
生效 #101553 (Immunofluorescence)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Immunostaining of human U-373 glioblastoma cells with ABIN524426 (red, A). Plasma membranes (green, B) and nuclei (blue, C) are visualized through overexpression of a CSPG4-GFP fusion protein and DAPI. D shows a merged image of the different channels. Z-projection of image taken at 63x]()
Immunostaining of human U-373 glioblastoma cells with ABIN524426 (red, A). Plasma membranes (green, B) and nuclei (blue, C) are visualized through overexpression of a CSPG4-GFP fusion protein and DAPI. D shows a merged image of the different channels. Z-projection of image taken at 63x
Full Methods -
- 缓冲液
- In 1x PBS, pH 7.4
- 注意事项
- Aliquot to avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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- 抗原
- STON1 (Stonin 1 (STON1))
- 别名
- STON1 (STON1 产品)
- 别名
- SALF antibody, SBLF antibody, STN1 antibody, STNB1 antibody, DKFZP469K176 antibody, 4921524J06Rik antibody, C86231 antibody, Salf antibody, Sblf antibody, stonin 1 antibody, STON1 antibody, ston1 antibody, Ston1 antibody
- 背景
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Full Gene Name: stonin 1
Synonyms: DKFZp781K2462,MGC149803,MGC149804,SBLF,STN1,STNB1,stoned-b1 - 基因ID
- 11037
- NCBI登录号
- NM_006873
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