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SPHK1 抗体 (AA 1-384)

Name: SPHK1 抗体 (AA 1-384)
SKU: ABIN522355
Availability: OutOfStock

This anti-SPHK1 antibody is a 小鼠单克隆 antibody detecting SPHK1 in WB 和 ELISA. Suitable for 人. This Primary Antibody has been cited in 11+ publications.

Western Blot analysis of SPHK1 expression in transfected 293T cell line by SPHK1 monoclonal antibody (M01), clone 1D6. (SPHK1 抗体 (AA 1-384))

Detection limit for recombinant GST tagged SPHK1 is approximately 1ng/ml as a capture antibody. (SPHK1 抗体 (AA 1-384))

ELISA

SPHK1 monoclonal antibody (M01), clone 1D6. (SPHK1 抗体 (AA 1-384))

Western Blot detection against Immunogen (67. (SPHK1 抗体 (AA 1-384))

5 images

(11 references)

产品编号 ABIN522355

发货至: 中国

Datasheet as PDF

Quick Overview for SPHK1 抗体 (AA 1-384) (ABIN522355)

抗原

See all SPHK1 抗体

SPHK1 (Sphingosine Kinase 1 (SPHK1))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This SPHK1 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA

克隆位点

1D6

抗原表位
15

9

7

7

7

6

6

5

5

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-384

原理

Mouse monoclonal antibody raised against a full length recombinant SPHK1.

序列

MDPAGGPRGV LPRPCRVLVL LNPRGGKGKA LQLFRSHVQP LLAEAEISFT LMLTERRNHA RELVRSEELG RWDALVVMSG DGLMHEVVNG LMERPDWETA IQKPLCSLPA GSGNALAASL NHYAGYEQVT NEDLLTNCTL LLCRRLLSPM NLLSLHTASG LRLFSVLSLA WGFIADVDLE SEKYRRLGEM RFTLGTFLLL AALRTYRGRL AYLPVGRVGS KTPASPVVVQ QGPVDAHLVP LEEPVPSHWT VVPDEDFVLV LALLHSHLGS EMFAAPMGRC AAGVMHLFYV RAGVSRAMLL RLFLAMEKGR HMEYECPYLV YVPVVAFRLE PKDGKGVFAV DGELMVSEAV QGQVHPNYFW MVSGCVEPPP SWKPQQMPPP EEPL

交叉反应

人

产品特性

Antibody Reactive Against Recombinant Protein.

免疫原

SPHK1 (AAH08040, 1 a.a. ~ 384 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

亚型

IgG2a
anti-Sphingosine Kinase 1 (SPHK1) (AA 286-315) antibody
SPHK1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB03621 unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 313-343) antibody
SPHK1 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 286-315) antibody
SPHK1 适用: 人, 小鼠, 大鼠, 灵长类 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 148-398) antibody
SPHK1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 1-384) antibody
SPHK1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 50-384) antibody
SPHK1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 1-30), (N-Term) antibody
SPHK1 适用: 人 WB 宿主: 兔 Polyclonal RB3619 unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 59-89) antibody
SPHK1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (AA 1-30) antibody
SPHK1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Sphingosine Kinase 1 (SPHK1) (C-Term) antibody
SPHK1 适用: 人 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
缓冲液

In 1x PBS, pH 7.4

注意事项

Aliquot to avoid repeated freezing and thawing.

储存条件

-20 °C

储存方法

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Sheu, Lee, Hua, Li, Lai, Lee, Cheng, Chen, Chan, Chao, Chen, Chang, Lee: "LRIG1 modulates aggressiveness of head and neck cancers by regulating EGFR-MAPK-SPHK1 signaling and extracellular matrix remodeling." in: Oncogene, Vol. 33, Issue 11, pp. 1375-84, (2014) (PubMed).

Böhm, Flößer, Ermler, Fender, Lüth, Kleuser, Schrör, Rauch: "Factor-Xa-induced mitogenesis and migration require sphingosine kinase activity and S1P formation in human vascular smooth muscle cells." in: Cardiovascular research, Vol. 99, Issue 3, pp. 505-13, (2013) (PubMed).

Zhang, Wang, Zhao, Di: "MiR-124 inhibits the migration and invasion of ovarian cancer cells by targeting SphK1." in: Journal of ovarian research, Vol. 6, Issue 1, pp. 84, (2013) (PubMed).

Schenten, Melchior, Steinckwich, Tschirhart, Bréchard: "Sphingosine kinases regulate NOX2 activity via p38 MAPK-dependent translocation of S100A8/A9." in: Journal of leukocyte biology, Vol. 89, Issue 4, pp. 587-96, (2011) (PubMed).

Ali-Rahmani, Hengst, Connor, Schengrund: "Effect of HFE variants on sphingolipid expression by SH-SY5Y human neuroblastoma cells." in: Neurochemical research, Vol. 36, Issue 9, pp. 1687-96, (2011) (PubMed).

Asher, Warren, Shaw, Sowter, Bali, Khan: "The role of Eag and HERG channels in cell proliferation and apoptotic cell death in SK-OV-3 ovarian cancer cell line." in: Cancer cell international, Vol. 11, pp. 6, (2011) (PubMed).

Schröder, Richter, Juan, Maltusch, Giegold, Quintini, Pfeilschifter, Huwiler, Radeke: "The sphingosine kinase 1 and S1P1 axis specifically counteracts LPS-induced IL-12p70 production in immune cells of the spleen." in: Molecular immunology, Vol. 48, Issue 9-10, pp. 1139-48, (2011) (PubMed).

Sinha, Schorn, Hochstim, Chinn, Zhu, Masood: "Increased radiation sensitivity of head and neck squamous cell carcinoma with sphingosine kinase 1 inhibition." in: Head & neck, Vol. 33, Issue 2, pp. 178-88, (2011) (PubMed).

Wang, Zhang, Lin, Xu, Feng, Ding: "A polysaccharide, MDG-1, induces S1P1 and bFGF expression and augments survival and angiogenesis in the ischemic heart." in: Glycobiology, Vol. 20, Issue 4, pp. 473-84, (2010) (PubMed).

Billich, Urtz, Reuschel, Baumruker: "Sphingosine kinase 1 is essential for proteinase-activated receptor-1 signalling in epithelial and endothelial cells." in: The international journal of biochemistry & cell biology, Vol. 41, Issue 7, pp. 1547-55, (2009) (PubMed).

in: (PubMed).
抗原

SPHK1 (Sphingosine Kinase 1 (SPHK1))

别名

SPHK1

背景

Full Gene Name: sphingosine kinase 1
Synonyms: SPHK

基因ID

8877

途径

VEGF Signaling