CD16 抗体
(4 references)Quick Overview for CD16 抗体 (ABIN509571)
抗原
See all CD16 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Hu CD16 Purified
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特异性
- The mouse monoclonal antibody 3G8 recognizes an extracellular epitope of CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA, 50-65 kDa, expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB, 48 kDa, mainly expressed on neutrophils). Regarding CD16-158V/F polymorphism, the antibody 3G8 detects both 158V and 158F allotype on natural NK cells.
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交叉反应 （详细）
- Human, Non-Human Primates
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Human neutrophils
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亚型
- IgG1 kappa
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anti-CD16 (CD16) antibody
CD16 适用: 人, Non-Human Primate FACS, IP 宿主: 小鼠 Monoclonal LNK16 unconjugated
anti-CD16 (CD16) antibody (FITC)CD16 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal LNK16 FITC
anti-CD16 (CD16) antibodyCD16 适用: 人 FACS, IP, WB, Func 宿主: 小鼠 Monoclonal MEM-154 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人, Pig, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-168 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人 FACS, IP, WB 宿主: 小鼠 Monoclonal MEM-154 unconjugated
anti-CD16 (CD16) antibody (Biotin)CD16 适用: 人 FACS, IP, WB 宿主: 小鼠 Monoclonal MEM-154 Biotin
anti-CD16 (CD16) (AA 17-208) antibodyCD16 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 2G10A9 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人, Non-Human Primate FACS, IP, Func, IHC (fro) 宿主: 小鼠 Monoclonal 3G8 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人, Pig, 绵羊, Cow, 马 FACS, Func 宿主: 小鼠 Monoclonal KD1 unconjugated
anti-CD16 (CD16) antibodyCD16 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal 2B1 unconjugated
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应用备注
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Flow cytometry: Recommended dilution: 6 μg/mL.
Immunohistochemistry (frozen sections): Acetone fixation. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Use of an anti-CD16 antibody for in vivo depletion of natural killer cells in rhesus macaques." in: Immunology, Vol. 124, Issue 2, pp. 215-22, (2008) (PubMed).
: "Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype." in: Cancer research, Vol. 68, Issue 4, pp. 976-80, (2008) (PubMed).
: "Down-regulation of activating Fcgamma receptors on monocytes of patients with rheumatoid arthritis upon methotrexate treatment." in: Rheumatology (Oxford, England), Vol. 44, Issue 6, pp. 729-34, (2005) (PubMed).
: "Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene." in: Blood, Vol. 91, Issue 7, pp. 2369-80, (1998) (PubMed).
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: "Use of an anti-CD16 antibody for in vivo depletion of natural killer cells in rhesus macaques." in: Immunology, Vol. 124, Issue 2, pp. 215-22, (2008) (PubMed).
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- CD16
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别名
- CD16
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背景
- CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms –, FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.,FcgammaRIII, IGFR3, FCRIII
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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