Dityrosine 抗体 (HRP)
Quick Overview for Dityrosine 抗体 (HRP) (ABIN5067464)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.
-
纯化方法
- Protein G Purified
-
免疫原
- Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
-
亚型
- IgG1
-
-
anti-Dityrosine (DT) antibody (Atto 594)
DT WB, ELISA, ICC, IF, FACS 宿主: 小鼠 Monoclonal 10A6 Atto 594
anti-Dityrosine (DT) antibodyDT WB, ELISA, ICC, IF, FACS 宿主: 小鼠 Monoclonal 10A6 unconjugated
anti-Dityrosine (DT) antibody (HRP)DT WB, ELISA, ICC, IF, FACS 宿主: 小鼠 Monoclonal 10A6 HRP
anti-Dityrosine (DT) antibody (FITC)DT WB, ELISA, ICC, IF, FACS 宿主: 小鼠 Monoclonal 10A6 FITC
anti-Dityrosine (DT) antibody (Biotin)DT WB, ELISA, ICC, IF, FACS 宿主: 小鼠 Monoclonal 10A6 Biotin
anti-Dityrosine (DT) antibody (PE)DT WB, ELISA, ICC, IF, FACS 宿主: 小鼠 Monoclonal 10A6 PE
anti-Dityrosine (DT) antibody (PerCP)DT WB, ELISA, ICC, IF, FACS 宿主: 小鼠 Monoclonal 10A6 PerCP
anti-Dityrosine (DT) antibody (APC)DT WB, ELISA, ICC, IF, FACS 宿主: 小鼠 Monoclonal 10A6 APC
anti-Dityrosine (DT) antibody (Atto 488)DT WB, ELISA, ICC, IF, FACS 宿主: 小鼠 Monoclonal 10A6 Atto 488
anti-Dityrosine (DT) antibody (Atto 390)DT WB, ELISA, ICC, IF, FACS 宿主: 小鼠 Monoclonal 10A6 Atto 390
-
-
应用备注
-
- WB (1:1000)
- ICC/IF (1:50)
- ELISA (1:1000)
- optimal dilutions for assays should be determined by the user.
-
说明
-
A 1:1000 dilution of ABIN5067464 was sufficient for detection of dityrosine in 1 μg of Dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Conjugated antibodies should be stored at 4°C
-
-
- Dityrosine (DT)
-
别名
- Dityrosine
-
物质类
- Dipeptide
-
背景
- ROS such as hydrogen peroxide (H2O2), superoxide, and hydroxyl radicals can react with both the backbone and the side chains of proteins, leading to backbone cleavage and side-chain modifications, respectively. Peroxidases, UV radiation, and hydroxyl radicals catalyze the formation of tyrosyl radicals which then react to form cross-links between proteins (1). This produces dityrosine, a metabolically stable biomarker of protein oxidation (2).
抗原
-
