Methylglyoxal (MG) 抗体 (Atto 488)
Quick Overview for Methylglyoxal (MG) 抗体 (Atto 488) (ABIN5067381)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, MDA, 4-HHE, 4-HNE, or Crotonaldehyde modified proteins.
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纯化方法
- Protein G Purified
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免疫原
- Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG2a
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anti-Methylglyoxal (MG) antibody
ELISA, WB, ICC, FACS, IF, IHC 宿主: 小鼠 Monoclonal 9E7 unconjugated
anti-Methylglyoxal (MG) antibody (HRP)ELISA, WB, ICC, FACS, IF, IHC 宿主: 小鼠 Monoclonal 9E7 HRP
anti-Methylglyoxal (MG) antibody (FITC)ELISA, WB, ICC, FACS, IF, IHC 宿主: 小鼠 Monoclonal 9E7 FITC
anti-Methylglyoxal (MG) antibody (Biotin)ELISA, WB, ICC, FACS, IF, IHC 宿主: 小鼠 Monoclonal 9E7 Biotin
anti-Methylglyoxal (MG) antibody (PE)ELISA, WB, ICC, FACS, IF, IHC 宿主: 小鼠 Monoclonal 9E7 PE
anti-Methylglyoxal (MG) antibody (PerCP)ELISA, WB, ICC, FACS, IF, IHC 宿主: 小鼠 Monoclonal 9E7 PerCP
anti-Methylglyoxal (MG) antibody (APC)ELISA, WB, ICC, FACS, IF, IHC 宿主: 小鼠 Monoclonal 9E7 APC
anti-Methylglyoxal (MG) antibody (Atto 594)ELISA, WB, ICC, FACS, IF, IHC 宿主: 小鼠 Monoclonal 9E7 Atto 594
anti-Methylglyoxal (MG) antibody (Atto 390)ELISA, WB, ICC, FACS, IF, IHC 宿主: 小鼠 Monoclonal 9E7 Atto 390
anti-Methylglyoxal (MG) antibodyELISA, WB, ICC, FACS, IF 宿主: 小鼠 Monoclonal 9F11 unconjugated
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应用备注
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- WB (1:1000)
- ICC/IF (1:50)
- FACS (1:50)
- FCM (1:50)
- ELISA (1:1000)
- IHC (1:50)
- optimal dilutions for assays should be determined by the user.
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说明
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A 1:1000 dilution of ABIN5067381 was sufficient for detection of Methylglyoxal in 0.5 μg of Methylglyoxal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- Methylglyoxal (MG)
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别名
- Methylglyoxal
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物质类
- Chemical
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背景
- Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Methylglyoxal is an alpha-oxoaldehyde generated from lipid peroxidation that reacts with proteins to form adducts(1). It reacts with free amino acids and protein residues to generate advanced glycation endproducts (AGEs) and crosslinks DNA polymerase and substrate DNA to severely inhibit DNA replication (2).
抗原
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