7-Ketocholesterol (7-KC) 抗体 (Atto 488)
Quick Overview for 7-Ketocholesterol (7-KC) 抗体 (Atto 488) (ABIN5067309)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Specific for 7-ketocholesterol modified proteins.
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纯化方法
- Protein G Purified
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免疫原
- Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG2b
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anti-7-Ketocholesterol (7-KC) antibody
ELISA, ICC, IF, WB 宿主: 小鼠 Monoclonal 7E1 unconjugated
anti-7-Ketocholesterol (7-KC) antibody (HRP)ELISA, ICC, IF, WB 宿主: 小鼠 Monoclonal 7E1 HRP
anti-7-Ketocholesterol (7-KC) antibody (FITC)ELISA, ICC, IF, WB 宿主: 小鼠 Monoclonal 7E1 FITC
anti-7-Ketocholesterol (7-KC) antibody (Biotin)ELISA, ICC, IF, WB 宿主: 小鼠 Monoclonal 7E1 Biotin
anti-7-Ketocholesterol (7-KC) antibody (PE)ELISA, ICC, IF, WB 宿主: 小鼠 Monoclonal 7E1 PE
anti-7-Ketocholesterol (7-KC) antibody (APC)ELISA, ICC, IF, WB 宿主: 小鼠 Monoclonal 7E1 APC
anti-7-Ketocholesterol (7-KC) antibody (PerCP)ELISA, ICC, IF, WB 宿主: 小鼠 Monoclonal 7E1 PerCP
anti-7-Ketocholesterol (7-KC) antibody (Atto 594)ELISA, ICC, IF, WB 宿主: 小鼠 Monoclonal 7E1 Atto 594
anti-7-Ketocholesterol (7-KC) antibody (Atto 390)ELISA, ICC, IF, WB 宿主: 小鼠 Monoclonal 7E1 Atto 390
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应用备注
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- WB (1:1000)
- ICC/IF (1:50)
- ELISA (1:1000)
- optimal dilutions for assays should be determined by the user.
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说明
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A 1:1000 dilution of ABIN5067309 was sufficient for detection of 7-Ketocholesterol in 2 μg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- 7-Ketocholesterol (7-KC)
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别名
- 7-Ketocholesterol
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物质类
- Hormone
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背景
- Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 7-ketocholesterol (7-KC) is a biomarker of lipoprotein cholesterol oxidation.
抗原
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