HNE 抗体 (HRP)
Quick Overview for HNE 抗体 (HRP) (ABIN5067302)
抗原
See all HNE 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Specific for 4-HNE modified proteins. Does not detect free 4-Hydroxynonenal. Does not X-react with 4-HHE, Acrolein, Crotonaldehyde, Hexanoyl Lysine, MDA, or Methylglyoxal modified proteins.
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纯化方法
- Protein G Purified
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免疫原
- Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG1
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anti-4-Hydroxynonenal (HNE) antibody
HNE WB, ELISA, IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-4-Hydroxynonenal (HNE) antibodyHNE WB, ELISA, IHC 宿主: 小鼠 Monoclonal HNEJ-2 unconjugated
anti-4-Hydroxynonenal (HNE) antibodyHNE WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 12F7 unconjugated
anti-4-Hydroxynonenal (HNE) antibody (FITC)HNE WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 12F7 FITC
anti-4-Hydroxynonenal (HNE) antibody (Biotin)HNE WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 12F7 Biotin
anti-4-Hydroxynonenal (HNE) antibody (PE)HNE WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 12F7 PE
anti-4-Hydroxynonenal (HNE) antibody (PerCP)HNE WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 12F7 PerCP
anti-4-Hydroxynonenal (HNE) antibody (APC)HNE WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 12F7 APC
anti-4-Hydroxynonenal (HNE) antibody (Atto 594)HNE WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 12F7 Atto 594
anti-4-Hydroxynonenal (HNE) antibody (Atto 488)HNE WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 12F7 Atto 488
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应用备注
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- WB (1:1000)
- ICC/IF (1:50)
- ELISA (1:1000)
- optimal dilutions for assays should be determined by the user.
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说明
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A 1:1000 dilution of ABIN5067302 was sufficient for detection of 4-Hydroxynonenal in 0.5 μg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- HNE (4-Hydroxynonenal (HNE))
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别名
- 4-Hydroxynonenal
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物质类
- Chemical
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背景
- 4-Hydroxy-2-nonenol (4-HNE) is an unsaturated aldehyde derived from lipid peroxidation. 4-HNE is an electrophile and reacts with protein nucleophiles such as cysteine, histine, and lysine (1). Low levels of 4-HNE promote cell survival via cellular antioxidant induction whereas higher levels lead to autophagy, apoptosis, and ultimately necrosis. 4-HNE has been linked to Alzheimer's disease, Parkinson's disease, cancer, cardiovascular diseases, diabetes, and liver disease.
抗原
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