Hexanoyl-Lysine Adduct (HEL) 抗体 (Biotin)
Quick Overview for Hexanoyl-Lysine Adduct (HEL) 抗体 (Biotin) (ABIN5067246)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
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纯化方法
- Protein G Purified
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免疫原
- Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG1
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应用备注
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- WB (1:1000)
- ICC/IF (1:50)
- ELISA (1:1000)
- FACS (1:50)
- FCM (1:50)
- optimal dilutions for assays should be determined by the user.
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说明
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A 1:1000 dilution of ABIN5067246 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 μg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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- Hexanoyl-Lysine Adduct (HEL)
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别名
- Hexanoyl-Lysine adduct
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背景
- Hexanoyl-lysine adduct (HEL) is a lysine adduct of 13-HPODE and is produced by the oxidation of omega-6 polyunsaturated fatty acids (1). It is a biomarker for the initial stage of lipid peroxidation. Lipid peroxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging.
抗原
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