Arginylation (N-Term) 抗体 (FITC)
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Quick Overview for Arginylation (N-Term) 抗体 (FITC) (ABIN5066977)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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特异性
- Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.
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纯化方法
- Protein G Purified
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免疫原
- Synthetic N-terminal arginylated peptide conjugated to KLH
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亚型
- IgG1
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anti-Arginylation (N-Term) antibody
WB, FACS 宿主: 小鼠 Monoclonal 4A9 unconjugated
anti-Arginylation (N-Term) antibody (HRP)WB, FACS 宿主: 小鼠 Monoclonal 4A9 HRP
anti-Arginylation (N-Term) antibody (Biotin)WB, FACS 宿主: 小鼠 Monoclonal 4A9 Biotin
anti-Arginylation (N-Term) antibody (PE)WB, FACS 宿主: 小鼠 Monoclonal 4A9 PE
anti-Arginylation (N-Term) antibody (APC)WB, FACS 宿主: 小鼠 Monoclonal 4A9 APC
anti-Arginylation (N-Term) antibody (PerCP)WB, FACS 宿主: 小鼠 Monoclonal 4A9 PerCP
anti-Arginylation (N-Term) antibody (Atto 594)WB, FACS 宿主: 小鼠 Monoclonal 4A9 Atto 594
anti-Arginylation (N-Term) antibody (Atto 488)WB, FACS 宿主: 小鼠 Monoclonal 4A9 Atto 488
anti-Arginylation (N-Term) antibody (Atto 390)WB, FACS 宿主: 小鼠 Monoclonal 4A9 Atto 390
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应用备注
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- WB (1:1000)
- FCM (1:50)
- optimal dilutions for assays should be determined by the user.
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说明
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A 1:1000 dilution of ABIN5066977 was sufficient for detection of N-terminal Arginylation in 0.5 ug of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- Arginylation
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物质类
- Chemical
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背景
- Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood (1). Arginylation has also been shown to regulate cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress (2).
抗原
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