BRD4 抗体 (AA 140-500)
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北京 101111
Quick Overview for BRD4 抗体 (AA 140-500) (ABIN5027061)
抗原
See all BRD4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 140-500
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纯化方法
- Protein A Chromatography
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免疫原
- A partial length recombinant BRD4 protein (amino acids 140-500) was used as the immunogen for this antibody.
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亚型
- IgG
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应用备注
- WB: 2-4 μg/mL This antibody detects ~80 kDa band. The size corresponds to Isoform 2 (Uniprot ID-O60885-2) of BRD4, HUNK1, the right size is ~152 kDa O60885 O60885
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- PBS containing 0.05 % BSA, PH 7.4
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
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- BRD4 (Bromodomain Containing 4 (BRD4))
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别名
- BRD4, HUNK1
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背景
- BRD4 is a member of the BET (bromo domain and extra terminal domain) family, along with BRD2, BRD3 and BRDT. BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. A chromosomal aberration involving BRD4 is found in a rare, aggressive and lethal carcinoma arising in midline organs of young people. It expressed ubiquitously and has three isoforms. Isoform B acts as a chromatin insulator in the DNA damage response pathway.
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分子量
- 88 kDa
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基因ID
- 23476
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UniProt
- O60885
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途径
- Chromatin Binding, SARS-CoV-2 Protein Interactome
抗原
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