WB: 2-4 μg/mL This antibody detects ~80 kDa band. The size corresponds to Isoform 2 (Uniprot ID-O60885-2) of BRD4, HUNK1, the right size is ~152 kDa O60885 O60885
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
PBS containing 0.05 % BSA, PH 7.4
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
抗原
BRD4
(Bromodomain Containing 4 (BRD4))
别名
BRD4, HUNK1
背景
BRD4 is a member of the BET (bromo domain and extra terminal domain) family, along with BRD2, BRD3 and BRDT. BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. A chromosomal aberration involving BRD4 is found in a rare, aggressive and lethal carcinoma arising in midline organs of young people. It expressed ubiquitously and has three isoforms. Isoform B acts as a chromatin insulator in the DNA damage response pathway.