Ubiquitin 抗体 (HRP)
Quick Overview for Ubiquitin 抗体 (HRP) (ABIN5024527)
抗原
See all Ubiquitin (Ub) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Detects ubiquitinated proteins and ubiquitin chains. Does not detect free ubiquitin.
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纯化方法
- Protein A Purified
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免疫原
- Ubiquitin conjugated lysozyme
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亚型
- IgG1
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应用备注
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- WB (1:10000)
- optimal dilutions for assays should be determined by the user.
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说明
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A 1:5000 dilution of ABIN5024527 was sufficient for detection of ubiquitin conjugates in 2.5 μg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 10 mg/mL
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缓冲液
- PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- Ubiquitin (Ub)
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别名
- Ubiquitin
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背景
- Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
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基因ID
- 7314
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UniProt
- P0CG47
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途径
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
抗原
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