Ubiquitin 抗体 (Biotin)
Quick Overview for Ubiquitin 抗体 (Biotin) (ABIN5024521)
抗原
See all Ubiquitin (Ub) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Detects ubiquitinated proteins and ubiquitin chains. Does not detect free ubiquitin.
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纯化方法
- Protein A Purified
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免疫原
- Ubiquitin conjugated lysozyme
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亚型
- IgG1
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anti-Ubiquitin (Ub) antibody
Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ub) (AA 1-229) antibodyUb 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, 犬, 马 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ub) (AA 1-229) antibodyUb 适用: 人, 大鼠 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal D2 unconjugated
anti-Ubiquitin (Ub) (acLys27) antibodyUb 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ub) antibody (HRP)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal HRP
anti-Ubiquitin (Ub) antibody (FITC)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal FITC
anti-Ubiquitin (Ub) antibody (Biotin)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal Biotin
anti-Ubiquitin (Ub) antibody (PE)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal PE
anti-Ubiquitin (Ub) antibody (APC)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal APC
anti-Ubiquitin (Ub) antibody (PerCP)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal PerCP
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应用备注
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- WB (1:10000)
- optimal dilutions for assays should be determined by the user.
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说明
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A 1:5000 dilution of ABIN5024521 was sufficient for detection of ubiquitin conjugates in 2.5 μg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 10 mg/mL
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缓冲液
- 10 mM phosphate buffer, 0.15M NaCl pH 7.4, 0.1 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- Ubiquitin (Ub)
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别名
- Ubiquitin
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背景
- Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
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基因ID
- 7314
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UniProt
- P0CG47
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途径
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
抗原
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