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Ubiquitin 抗体 (APC)

Ubiquitin 适用: 人 WB, ELISA, IHC, IF, ICC 宿主: 小鼠 Monoclonal FK1 APC
产品编号 ABIN5024464
发货至: 中国
  • 抗原 See all Ubiquitin 抗体
    Ubiquitin
    适用
    • 141
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    宿主
    • 99
    • 68
    • 3
    • 1
    • 1
    小鼠
    克隆类型
    • 92
    • 80
    单克隆
    标记
    • 73
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    This Ubiquitin antibody is conjugated to APC
    应用范围
    • 156
    • 85
    • 79
    • 59
    • 53
    • 41
    • 27
    • 21
    • 20
    • 14
    • 13
    • 9
    • 4
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    特异性
    Detects polyubiquitinated proteins and ubiquitin chains. Does not detect monoubiquitinated proteins nor free ubiquitin.
    纯化方法
    Gel filtration
    免疫原
    Ubiquitin conjugated lysozyme
    克隆位点
    FK1
    亚型
    IgM
    Top Product
    Discover our top product Ubiquitin Primary Antibody
  • 应用备注
    • WB (1:10000)
    • optimal dilutions for assays should be determined by the user.
    说明

    A 1:5000 dilution of SMC-213 was sufficient for detection of ubiquitin conjugates in 2.5 μg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    10 mM phosphate buffer, 0.15M NaCl pH 7.4, 0.1 % sodium azide, Storage buffer may change when conjugated
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Conjugated antibodies should be stored at 4°C
  • 抗原
    Ubiquitin
    别名
    Ubiquitin (Ubiquitin 产品)
    背景
    Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
    基因ID
    7314
    UniProt
    P0CG47
    途径
    Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
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