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Ubiquitin 抗体 (Atto 594)

This anti-Ubiquitin antibody is a 小鼠 单克隆 antibody detecting Ubiquitin in WB, IHC, ELISA, ICC 和 IF. Suitable for 人.
产品编号 ABIN5024446
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Ubiquitin 抗体 (Atto 594) (ABIN5024446)

抗原

See all Ubiquitin (Ub) 抗体
Ubiquitin (Ub)

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Ubiquitin antibody is conjugated to Atto 594

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

克隆位点

FK1
  • 特异性

    Detects polyubiquitinated proteins and ubiquitin chains. Does not detect monoubiquitinated proteins nor free ubiquitin.

    纯化方法

    Gel filtration

    免疫原

    Ubiquitin conjugated lysozyme

    亚型

    IgM
  • 应用备注

    • WB (1:10000)
    • optimal dilutions for assays should be determined by the user.

    说明

    A 1:5000 dilution of SMC-213 was sufficient for detection of ubiquitin conjugates in 2.5 μg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    10 mM phosphate buffer, 0.15M NaCl pH 7.4, 0.1 % sodium azide, Storage buffer may change when conjugated

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Conjugated antibodies should be stored at 4°C
  • 抗原

    Ubiquitin (Ub)

    别名

    Ubiquitin

    背景

    Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.

    基因ID

    7314

    UniProt

    P0CG47

    途径

    Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
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