SKA3 抗体 (C-Term)
Quick Overview for SKA3 抗体 (C-Term) (ABIN500732)
抗原
See all SKA3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- This antibody detects SKA3.
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交叉反应 （详细）
- Species reactivity (tested):Human, mouse, rat
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纯化方法
- Peptide affinity chromatography
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免疫原
- SKA3 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of human SKA3.
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亚型
- IgG
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anti-Spindle and Kinetochore Associated Complex Subunit 3 (SKA3) (AA 201-300) antibody
SKA3 适用: 人, 大鼠 ELISA, WB, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Spindle and Kinetochore Associated Complex Subunit 3 (SKA3) (AA 101-195) antibodySKA3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Spindle and Kinetochore Associated Complex Subunit 3 (SKA3) (C-Term) antibodySKA3 适用: 人, 大鼠, 小鼠 ELISA, WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Spindle and Kinetochore Associated Complex Subunit 3 (SKA3) (AA 226-253) antibodySKA3 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB23058 unconjugated
anti-Spindle and Kinetochore Associated Complex Subunit 3 (SKA3) (Internal Region) antibodyVerified SKA3 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Spindle and Kinetochore Associated Complex Subunit 3 (SKA3) (AA 323-372) antibodySKA3 适用: 人, 犬, 豚鼠, 马, 猴, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA. Western blot: 0.5 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- PBS containing 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- SKA3 (Spindle and Kinetochore Associated Complex Subunit 3 (SKA3))
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别名
- SKA3
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背景
- Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis.Synonyms: C13orf3, RAMA1, Spindle and kinetochore-associated protein 3
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基因ID
- 221150
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NCBI登录号
- NP_659498
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UniProt
- Q8IX90
抗原
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