NOTUM 抗体 (N-Term)
This anti-NOTUM antibody is a 兔 多克隆 antibody detecting NOTUM in WB, IHC (p) 和 EIA. Suitable for 人, 小鼠 和 大鼠.
Quick Overview for NOTUM 抗体 (N-Term) (ABIN500375)
抗原
See all NOTUM 抗体
NOTUM
(Notum Pectinacetylesterase Homolog (NOTUM))
适用
人, 小鼠, 大鼠
宿主
兔
克隆类型
多克隆
标记
This NOTUM antibody is un-conjugated
应用范围
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
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抗原表位
- N-Term
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特异性
- This antibody detects Notum.
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交叉反应 （详细）
- Species reactivity (tested):Human, Mouse, Rat.
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纯化方法
- Peptide Affinity Chromatography
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免疫原
- NOTUM antibody was raised against a 19 amino acid peptide near the amino terminus of Human NOTUM.
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亚型
- IgG
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anti-Notum Pectinacetylesterase Homolog (NOTUM) (AA 100-235) antibody
NOTUM 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Notum Pectinacetylesterase Homolog (NOTUM) (AA 20-320) antibodyNOTUM 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS containing 0.02 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- NOTUM (Notum Pectinacetylesterase Homolog (NOTUM))
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别名
- Notum
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背景
- In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by b-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.
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基因ID
- 147111
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NCBI登录号
- NP_848588
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UniProt
- Q6P988
抗原
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