MED28 抗体 (Center)
This anti-MED28 antibody is a 兔 多克隆 antibody detecting MED28 in WB, IHC (p) 和 EIA. Suitable for 人 和 小鼠.
Quick Overview for MED28 抗体 (Center) (ABIN500268)
抗原
See all MED28 抗体
MED28
(Mediator Complex Subunit 28 (MED28))
适用
人, 小鼠
宿主
兔
克隆类型
多克隆
标记
This MED28 antibody is un-conjugated
应用范围
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
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抗原表位
- Center
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特异性
- This antibody detects MED28.
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交叉反应 （详细）
- Species reactivity (tested):Human, mouse
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纯化方法
- Peptide affinity chromatography
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免疫原
- MED28 antibody was raised against a 17 amino acid peptide from near the center of human MED28.
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亚型
- IgG
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anti-Mediator Complex Subunit 28 (MED28) (AA 1-178) antibody
MED28 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 28 (MED28) antibodyMED28 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- PBS containing 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- MED28 (Mediator Complex Subunit 28 (MED28))
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别名
- MED28
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背景
- The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED28, also known as Magicin. MED28 is expressed in many cell lines and tissues. It has been shown that a down-regulation of MED28 expression in NIH3T3 cells results in a significant induction of several genes associated with smooth muscle cell differentiation and conversely its over-expression represses expression of SMC genes. MED28 can also form a ternary complex with Grb2 and Merlin, the neurofibromatosis 2 tumor suppressor protein, indicating that MED28 may play a role in Merlin's tumor suppressive activity. MED28 has also been recently identified as an HIV dependency factor (HDF), suggesting that MED28 may be an important drug target in HIV treatment. At least two isoforms of MED28 are known to exist.Synonyms: EG1, Endothelial-derived protein 1, FKSG20, Mediator complex subunit 28, Mediator of RNA polymerase II transcription subunit 28, Merlin and Grb2-interacting cytoskeletal protein, Tumor angiogenesis marker EG-1
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基因ID
- 80306
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NCBI登录号
- NP_079481
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UniProt
- Q9H204
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途径
- Stem Cell Maintenance, Regulation of Muscle Cell Differentiation
抗原
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