PTK6 抗体 (pTyr447) (AbBy Fluor® 680)
Quick Overview for PTK6 抗体 (pTyr447) (AbBy Fluor® 680) (ABIN4997632)
抗原
See all PTK6 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr447
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交叉反应
- 人
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纯化方法
- Purified by Protein A.
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免疫原
- KLH conjugated synthetic phosphopeptide derived from human Brk around the phosphorylation site of Tyr447 [TS(p-Y)EN]
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亚型
- IgG
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anti-PTK6 Protein tyrosine Kinase 6 (PTK6) (AA 1-451) antibody
PTK6 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-PTK6 Protein tyrosine Kinase 6 (PTK6) (AA 222-451) antibodyPTK6 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-PTK6 Protein tyrosine Kinase 6 (PTK6) (AA 191-445) antibodyPTK6 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-PTK6 Protein tyrosine Kinase 6 (PTK6) (AA 1-113) antibodyPTK6 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-PTK6 Protein tyrosine Kinase 6 (PTK6) (AA 342-451) antibodyPTK6 适用: 人 ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2F11 unconjugated
anti-PTK6 Protein tyrosine Kinase 6 (PTK6) antibodyPTK6 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H12B8 unconjugated
anti-PTK6 Protein tyrosine Kinase 6 (PTK6) (AA 11-41), (N-Term) antibodyPTK6 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB3073-3074 unconjugated
anti-PTK6 Protein tyrosine Kinase 6 (PTK6) (AA 342-452) antibodyPTK6 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2F11 unconjugated
anti-PTK6 Protein tyrosine Kinase 6 (PTK6) (AA 287-336) antibodyPTK6 适用: 人, 马 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
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有效期
- 12 months
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- PTK6 (PTK6 Protein tyrosine Kinase 6 (PTK6))
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别名
- Brk
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背景
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Synonyms: BRK, Protein-tyrosine kinase 6, Breast tumor kinase, Tyrosine-protein kinase BRK, PTK6
Background: Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF, transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p19RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p19RhoGAP, which promotes association with RASA1/p12RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways. Isoform 2 inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins.
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基因ID
- 5753
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UniProt
- Q13882
抗原
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