BUB3 抗体 (C-Term, N-Term)
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北京 101111
Quick Overview for BUB3 抗体 (C-Term, N-Term) (ABIN499513)
抗原
See all BUB3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term, N-Term
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特异性
- This antibody reacts to Bub3.
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纯化方法
- Affinity chromatography purified via peptide column
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免疫原
- Bub3 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human bub3.
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亚型
- IgG
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应用备注
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ELISA. Western Blot: Bub3 antibody can be used for detection of bub3 at 0.5 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- PBS containing 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))
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别名
- BUB3
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背景
- The mitotic checkpoint protein Bub3 is involved with the essential spindle checkpoint pathway which operates during early embryogenesis. Bub3 is important during G2 and early mitosis stages, permitting entry into mitosis depending upon the assembly state of microtubules, thus preventing premature sister chromatid separation, mis-segregation and aneuploidy. Bub3 contains four WD repeat domains and is required for the kinetochore localization of Bub1, a related kinase that is necessary for spindle assembly checkpoint function. Bub1 is able to autophosphorylate and can catalyze the phosphorylation of Bub3. Both Bub1 and Bub3 are mutually dependent for function. Altered Bub expression levels may significantly impair mitotic checkpoint function and is associated with tumor cell proliferation.Synonyms: Mitotic checkpoint protein BUB3
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基因ID
- 9184
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UniProt
- O43684
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途径
- Maintenance of Protein Location
抗原
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