BUB1 抗体 (N-Term)
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Quick Overview for BUB1 抗体 (N-Term) (ABIN499509)
抗原
See all BUB1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- This antibody reacts to Bub1.
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纯化方法
- Affinity chromatography purified via peptide column
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免疫原
- Bub1 antibody was raised against a 19 amino acid peptide from near the amino terminus of human Bub1.
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亚型
- IgG
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应用备注
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ELISA. Western Blot: Bub1 antibody can be used for detection of bub1 at 2 - 4 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- PBS containing 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))
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别名
- BUB1A
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背景
- Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.Synonyms: BUB1L, Mitotic checkpoint serine/threonine-protein kinase BUB1
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基因ID
- 699
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UniProt
- O43683
抗原
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