EBF1 抗体 (Internal Region)
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Quick Overview for EBF1 抗体 (Internal Region) (ABIN499164)
抗原
See all EBF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- C-HNQLPALANT SV
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特异性
- This antibody detects EBF1 / COE1 (Internal region). It is expected not to cross-react with EBF2, EBF3 and EBF4.
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交叉反应 （详细）
- Species reactivity (expected):Mouse, ratSpecies reactivity (tested):Human
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from the internal region of the protein sequence according to NP_076870.1
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anti-Early B-Cell Factor 1 (EBF1) (AA 179-451) antibody
EBF1 适用: 人 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Early B-Cell Factor 1 (EBF1) (AA 1-591) antibodyEBF1 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 1G8 unconjugated
anti-Early B-Cell Factor 1 (EBF1) (AA 1-591) antibodyEBF1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1C12 unconjugated
anti-Early B-Cell Factor 1 (EBF1) (AA 1-560) antibodyEBF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Early B-Cell Factor 1 (EBF1) (N-Term) antibodyEBF1 适用: 人, 小鼠, 大鼠, Cow, 兔, 犬, 豚鼠, 马, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Early B-Cell Factor 1 (EBF1) (Internal Region) antibodyVerified EBF1 适用: 人, 小鼠 WB, ELISA, IP 宿主: 山羊 Polyclonal unconjugated
anti-Early B-Cell Factor 1 (EBF1) (AA 57-317) antibodyEBF1 适用: 人, 小鼠, Cow WB 宿主: 兔 Polyclonal unconjugated
anti-Early B-Cell Factor 1 (EBF1) (C-Term) antibodyEBF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Early B-Cell Factor 1 (EBF1) (AA 150-199) antibodyEBF1 适用: 人, 小鼠, 大鼠, Cow, 兔, 犬, 豚鼠, 马, 斑马鱼, 小鸡, Drosophila melanogaster, 猴, Pig, Bat, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Early B-Cell Factor 1 (EBF1) antibodyEBF1 适用: 人, 小鼠, 大鼠, Cow, 兔, 犬, 豚鼠, 马, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: limit dilution 1: 64000. Western blot: 0.3-0.1 μg/mL, approx.75 kDa band observed in Human Adipose lysates. Anonymous customer used this product successfully in IP from induced 3T3-L1 expressingFLAG-EBF1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.
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- EBF1 (Early B-Cell Factor 1 (EBF1))
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别名
- EBF1 / COE1
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背景
- EBF1 belongs to the COE family. It contains 1 IPT/TIG domain. EBF1 is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Forms either a homodimer or a heterodimer with a related family member. Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes.Synonyms: EBF, Early B-cell factor, OE-1, Transcription factor COE1
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基因ID
- 1879
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NCBI登录号
- NP_076870
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UniProt
- Q9UH73
抗原
-
