Tppp 抗体 (Internal Region)
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Quick Overview for Tppp 抗体 (Internal Region) (ABIN499122)
抗原
See all Tppp 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- C-DPSKDRAAKR LSLES
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特异性
- Recognizes Tubulin polymerization-promoting protein (TPPP).
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交叉反应 (详细)
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Species reactivity (expected):Porcine.
Species reactivity (tested):Human, Mouse and Rat. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence from the Internal region of the protein sequence according to NP_008961.1. Genename: TPPP
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应用备注
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Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.001-0.01 μg/mL. Detects a band of approx. 26 kDa in Human Brain (Cerebellum),Mouse and Rat Brain lysates. An additional band of unknown identity was also consistentlyobserved at 16 kDa. This band was successfully blocked by incubation with the immunizingpeptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Tppp (Tubulin Polymerization Promoting Protein (Tppp))
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别名
- TPPP
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背景
- Tubulin Polymerization Promoting Protein (TPPP) promotes in vitro polymerization of tubulin into double-walled tubules and polymorphic aggregates or bundled stabilized microtubules blocks. When overexpressed, TPPP inhibits mitotic spindle assembly and nuclear envelope breakdown, apparently without affecting other cellular events.Synonyms: 25 kDa brain-specific protein, TPPP1, Tubulin polymerization-promoting protein
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基因ID
- 11076
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NCBI登录号
- NP_008961
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UniProt
- O94811
抗原
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