EIF3D 抗体
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北京 101111
Quick Overview for EIF3D 抗体 (ABIN499071)
抗原
See all EIF3D 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of eIF3ζ protein. (region surrounding Arg135)
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交叉反应 (详细)
- Species reactivity (tested):Human, Mouse.
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纯化方法
- Affinity Chromatography using epitope-specific immunogen.
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应用备注
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Western blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- EIF3D (Eukaryotic Translation Initiation Factor 3, Subunit D (EIF3D))
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别名
- EIF3D / EIF3S7
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背景
- Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex containing methionyl initiator tRNA (Met-tRNAiMet), which is base paired at the initiation codon (AUG, GUG) in eligible transcripts. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Eukaryotic initiation factor 3 (eIF3) is the largest family of eIFs and consists of at least 12 unique subunits in mammals. eIFE, also known as eIF p47, binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA and associates with the complex p170-eIF3.Synonyms: Eukaryotic translation initiation factor 3 subunit 7, Eukaryotic translation initiation factor 3 subunit D, eIF-3-zeta, eIF3 p66
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分子量
- approx. 64 kDa
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基因ID
- 8664
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NCBI登录号
- NP_003744
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UniProt
- O15371
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途径
- Ribonucleoprotein Complex Subunit Organization
抗原
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