POLR2J 抗体
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Quick Overview for POLR2J 抗体 (ABIN499064)
抗原
See all POLR2J 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of POLR2J1 protein. (region surrounding Asp24)
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交叉反应 （详细）
- Species reactivity (tested):Human and Mouse.
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纯化方法
- Affinity chromatography using epitope-specific immunogen
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纯度
- > 95 % (by SDS-PAGE)
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anti-Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J) (AA 1-117) antibody
POLR2J 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J) (AA 1-117) antibodyPOLR2J 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A10 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J) (AA 86-115), (C-Term) antibodyPOLR2J 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB29029 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J) (AA 93-117), (C-Term) antibodyPOLR2J 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J) (N-Term) antibodyPOLR2J 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- POLR2J (Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J))
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别名
- POLR2J
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背景
- Four independent genes encoding various variants of the hRPB11 subunit of Homo sapiens RNA polymerase II were revealed in human chromosome 7. Three genes (POLR2 J1, POLR2 J2, and POLR2 J3) form a cluster of total length of 214 530 bp in the genetic locus 7q22.1 on the long arm of chromosome 7 (contig NT_007933). The fourth gene (POLR2 J4, 31 040 bp) was localized in the cytogenetic locus 7p13 of the short arm of chromosome 7 (contig NT_007819). An analysis enabled us to refine dissimilar experimental data on the mapping of the hRPB11 subunit gene on chromosome 7. In particular, the presence of three sites of its localization according to data on hybridization with fluorescent-labeled probes (the FISH method) was explained. It was established that, upon the expression of the four human POLR2 J genes, at least 14 types of mature mRNAs encoding somewhat differing hRPB11 isoforms can be synthesized. Eleven of these mRNAs were revealed (as full-length copies or clearly identifiable fragments) in the available databases of expressed sequence tags and cDNAs. The most probable scheme of origination of the multiple genes of the POLR2 J family as a result of three consecutive segmented duplications increasing in size was proposed and substantiated. On the basis of the scheme, some assumptions on the pathways of evolution of separate human genes and the mechanisms of generation of protein diversity in higher eukaryotes were made.Synonyms: DNA-directed RNA polymerase II subunit J-1, DNA-directed RNA polymerase II subunit RPB11-a, POLR2J1, RNA polymerase II 13.3 kDa subunit, RNA polymerase II subunit B11-a, RPB11a
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分子量
- approx. 13 kDa
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基因ID
- 5439
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NCBI登录号
- NP_006225
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UniProt
- P52435
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途径
- Regulatory RNA Pathways
抗原
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