AFT1 抗体
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Quick Overview for AFT1 抗体 (ABIN498985)
抗原
See all AFT1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of ATF-1 protein. (region surrounding Ile57)
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交叉反应 （详细）
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Species reactivity (expected):Mouse.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography
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纯度
- > 95 % by SDS-PAGE
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anti-Activating Transcription Factor 1 (AFT1) (AA 1-271) antibody
AFT1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 1-271) antibodyAFT1 适用: 人 WB, ELISA, IHC (p), RNAi 宿主: 小鼠 Monoclonal 3E7 unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 3-245) antibodyAFT1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 1-271) antibodyAFT1 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 1-271) antibodyAFT1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 1-271) antibodyAFT1 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (C-Term) antibodyAFT1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 3-213) antibodyAFT1 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (N-Term) antibodyAFT1 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 豚鼠, 猴, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 1-272) antibodyAFT1 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3E7 unconjugated
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应用备注
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- AFT1 (Activating Transcription Factor 1 (AFT1))
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别名
- ATF1
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背景
- Eukaryotic gene transcription is regulated by sequencespecific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/ CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-1 is shown to play a key role in the induction of NOX1, this protein binds the cAMP response element (CRE) and mediates PKAinduced stimulation of CRE-reporter genes. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CREdependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 functions as a histone acetyltransferase (HAT) and acetylates Histones H2B and H4 specifically in vitro.Synonyms: ATF-1, Activating transcription factor 1, Cyclic AMP-dependent transcription factor ATF-1, Protein TREB36, cAMP-dependent transcription factor ATF-1
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分子量
- approx. 29 kDa
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基因ID
- 466
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NCBI登录号
- NP_005162
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UniProt
- P18846
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途径
- Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades
抗原
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