POLR2E 抗体
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Quick Overview for POLR2E 抗体 (ABIN498959)
抗原
See all POLR2E 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of POLR2E protein. (region surrounding Leu58)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography
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纯度
- > 95 % by SDS-PAGE
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anti-Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E) (Internal Region) antibody
POLR2E 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E) antibodyPOLR2E 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB13230 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E) antibodyPOLR2E 适用: 人, 小鼠, 大鼠, 猴, 犬 WB, IHC, IF, FACS 宿主: 小鼠 Monoclonal 3B5 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E) (AA 1-210) antibodyPOLR2E 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E) (AA 1-210) antibodyPOLR2E 适用: 人 IP 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E) (Center) antibodyPOLR2E 适用: 人, 小鼠, 大鼠, 猴, Cow WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))
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别名
- POLR2E
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背景
- This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in twofold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.Synonyms: DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, DNA-directed RNA polymerases I, II, II, RNA polymerases I, RPABC1, RPB5 homolog, XAP4, and III subunit ABC1, and III subunit RPABC1
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分子量
- approx. 25 kDa
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基因ID
- 5434
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NCBI登录号
- NP_002686
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UniProt
- P19388
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途径
- Regulatory RNA Pathways
抗原
-
