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- 抗原 See all POLR2E 抗体
- POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This POLR2E antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- This antibody detects endogenous levels of POLR2E protein. (region surrounding Leu58)
- 交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - 纯化方法
- Affinity chromatography
- 纯度
- > 95 % by SDS-PAGE
- Top Product
- Discover our top product POLR2E Primary Antibody
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anti-Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E) (AA 1-210) antibody
POLR2E 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E) antibodyPOLR2E 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E) (Internal Region) antibodyPOLR2E 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E) antibodyPOLR2E 适用: 人, 小鼠, 大鼠, 猴, 犬 WB, IHC, IF, FACS 宿主: 小鼠 Monoclonal 3B5 unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E) (AA 1-210) antibodyPOLR2E 适用: 人 IP 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E) (AA 1-210) antibodyPOLR2E 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E) (Center) antibodyPOLR2E 适用: 人, 小鼠, 大鼠, 猴, Cow WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1,0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))
- 别名
- POLR2E (POLR2E 产品)
- 别名
- MGC68604 antibody, polr2e antibody, wu:fk97g10 antibody, zgc:101098 antibody, 2410021N14Rik antibody, 25kDa antibody, AW208866 antibody, RPB5 antibody, XAP4 antibody, RPABC1 antibody, hRPB25 antibody, hsRPB5 antibody, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa S homeolog antibody, RNA polymerase II subunit E antibody, polymerase (RNA) II (DNA directed) polypeptide E, b antibody, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa antibody, polymerase (RNA) II (DNA directed) polypeptide E antibody, DNA-directed RNA polymerases I, II, and III subunit RPABC1 antibody, polr2e.S antibody, POLR2E antibody, polr2eb antibody, polr2e antibody, Polr2e antibody, rpb-5 antibody
- 背景
- This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in twofold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.Synonyms: DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, DNA-directed RNA polymerases I, II, II, RNA polymerases I, RPABC1, RPB5 homolog, XAP4, and III subunit ABC1, and III subunit RPABC1
- 分子量
- approx. 25 kDa
- 基因ID
- 5434
- NCBI登录号
- NP_002686
- UniProt
- P19388
- 途径
- Regulatory RNA Pathways
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