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- 抗原 See all EIF4E 抗体
- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
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适用
- 人, 大鼠, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This EIF4E antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- This antibody detects endogenous levels of eIF4E protein. (region surrounding Arg215)
- 交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - 纯化方法
- Affinity chromatography
- 纯度
- > 95 % by SDS-PAGE
- Top Product
- Discover our top product EIF4E Primary Antibody
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anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 121-217) antibody
EIF4E 适用: 人, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 1-100) antibodyEIF4E 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) antibodyEIF4E 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5D11 unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (pSer209) antibodyEIF4E 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (C-Term) antibodyEIF4E 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) antibodyEIF4E 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (pSer209) antibodyEIF4E 适用: 人, 大鼠, 小鼠, 斑马鱼 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (C-Term) antibodyEIF4E 适用: 人, 大鼠, 小鼠, 斑马鱼, Cow, 兔, 犬, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (pSer209) antibodyEIF4E 适用: 人, 大鼠, 小鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 2B1 unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 2-217) antibodyEIF4E 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1,0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
- 别名
- EIF4E (EIF4E 产品)
- 背景
- EIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.Synonyms: EIF-4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein
- 分子量
- approx. 25 kDa
- 基因ID
- 1977
- NCBI登录号
- NP_001959
- UniProt
- P06730
- 途径
- BCR Signaling
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