FOXO1 抗体 (pSer256)
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Quick Overview for FOXO1 抗体 (pSer256) (ABIN498785)
抗原
See all FOXO1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer256
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特异性
- This antibody detects endogenous levels of p-FKHR/FOXO1A protein only when phosphorylated at Ser256 .
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography
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纯度
- > 95 % by SDS-PAGE
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anti-Forkhead Box O1 (FOXO1) (AA 165-270) antibody
FOXO1 适用: 人, 小鼠, 大鼠, Cow, Pig, 犬, 小鸡 WB, ELISA, IF (p), IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Forkhead Box O1 (FOXO1) (AA 333-557) antibodyFOXO1 适用: 人 WB, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Forkhead Box O1 (FOXO1) antibodyFOXO1 适用: 人, 小鼠 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 3B6 unconjugated
anti-Forkhead Box O1 (FOXO1) antibodyFOXO1 适用: 人 WB, IHC, ELISA, IF 宿主: 小鼠 Monoclonal 3B6 unconjugated
anti-Forkhead Box O1 (FOXO1) antibodyKD Validated FOXO1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB850 unconjugated Recombinant Antibody
anti-Forkhead Box O1 (FOXO1) (AA 556-655) antibodyFOXO1 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 4E9 unconjugated
anti-Forkhead Box O1 (FOXO1) (pSer256) antibodyFOXO1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Forkhead Box O1 (FOXO1) (AA 452-555) antibodyFOXO1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3A10 unconjugated
anti-Forkhead Box O1 (FOXO1) antibodyFOXO1 适用: 人, 小鼠 WB, IF, IHC (p), EIA 宿主: 小鼠 Monoclonal 3B6 unconjugated
anti-Forkhead Box O1 (FOXO1) (AA 222-469) antibodyFOXO1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- FOXO1 (Forkhead Box O1 (FOXO1))
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别名
- FOXO1A
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背景
- The transcription factor forkhead in rhabdomyosarcoma (FKHR), which is inhibited by insulin and IGF-1, enhances transcription. FKHR has been implicated in alveolar rhabdomyosarcoma, a soft tissue tumor wherein a chromosomal translocation [t(2,12)(q35,q14)] occurs between the FKHR and PAX3 genes, resulting in a novel chimeric protein with abnormal levels of expression. FKHR becomes phosphorylated at Ser 319, Ser 256 and Thr 256 by protein kinase B (PKB) in a phosphoinsoditide 3 (PI 3)-kinase/Akt-dependent pathway, resulting in the inactivation and subsequent nuclear exit of FKHR. In addition, FKHR becomes phosphorylated at Ser 329, also resulting in decreased FKHR activity and diminished nuclear FKHR concentration. However, phosphorylation of FKHR at Ser 329 is not mediated by a PI 3-kinasedependent pathway, but by an alternate mechanism. Dualspecificity tyrosine-phosphorylated and regulated kinase 1A (DYRK1A), which co-localizes to the same region of the nucleus as FKHR, specifically phosphorylates FKHR at Ser 329 in rabbit skeletal muscle.Synonyms: FKHR, FOXO1, Forkhead Box Protein O1, Forkhead Box Protein O1A, Forkhead in Rhabdomyosarcoma
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分子量
- approx. 70 to 82 kDa
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基因ID
- 2308
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NCBI登录号
- NP_002006
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UniProt
- Q12778
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途径
- PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling
抗原
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