MARCKS 抗体
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Quick Overview for MARCKS 抗体 (ABIN498653)
抗原
See all MARCKS 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of MARCKS protein. (region surrounding Arg157)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography
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纯度
- > 95 % by SDS-PAGE
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anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 2-65) antibody
MARCKS 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2C2 unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 1-100) antibodyMARCKS 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer170) antibodyMARCKS 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 2-66) antibodyMARCKS 适用: 人, 小鼠 WB, ELISA, IF, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2C2 unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (Internal Region) antibodyMARCKS 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer152), (Ser156) antibodyMARCKS 适用: 人, 小鼠, 大鼠, Cow, 小鸡, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer162) antibodyMARCKS 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer158) antibodyMARCKS 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 31-120) antibodyMARCKS 适用: 大鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer159) antibodyMARCKS 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
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别名
- MARCKS
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背景
- Myristoylated alanine-rich protein kinase C substrate (MARCKS), also designated 80K or 80K-L, has been identified as a major cellular substrate for protein kinase C. Human MARCKS is a 332 amino acid protein with a calculated molecular weight of 31.534 kDa, however, it has been shown to run at 80-87 kDa on Western blot. The plasma membrane bound protein dissociates from the membrane upon phosphorylation by various PKC isoforms. In NIH/3T3 fibroblasts, PKC alpha and PKC epsilon, but not PKC delta, are responsible for MARCKS phosphorylation. MARCKS has been found to bind calmodulin, Actin and Synapsin and is a filamentous (F) Actin crosslinking protein.Synonyms: MACS, Myristoylated alanine-rich C-kinase substrate, PRKCSL
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分子量
- approx. 32, 75, 87 kDa
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基因ID
- 4082
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NCBI登录号
- NP_002347
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UniProt
- P29966
抗原
-
