ZAP70 抗体
Our Local Distributor
北京 101111
Quick Overview for ZAP70 抗体 (ABIN498597)
抗原
See all ZAP70 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- This antibody detects endogenous levels of ZAP-70 protein. (region surrounding Ser313)
-
交叉反应 （详细）
-
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography using epitope-specific immunogen
-
纯度
- > 95 % (by SDS-PAGE)
-
-
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (C-Term) antibody
ZAP70 适用: 人 WB, ICC, ICFC 宿主: 小鼠 Monoclonal ZAP-03 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr319) antibodyZAP70 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 247-382) antibodyZAP70 适用: 人 IHC, ELISA, FACS, IF 宿主: 小鼠 Monoclonal ZAP70-2035 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 169-390) antibodyZAP70 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 1F3G9 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 337-600) antibodyZAP70 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibodyZAP70 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 1-254) antibodyZAP70 适用: 人 IHC, FACS, IF 宿主: 小鼠 Monoclonal 2F3-2 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 169-390) antibodyZAP70 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6D9C2 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 247-382) antibodyZAP70 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal ZAP70-2046 unconjugated
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibodyZAP70 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
-
-
应用备注
-
Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
-
-
- ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
-
别名
- ZAP70
-
背景
- The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR) which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti α and β chains, the CD3 γ, δ and ε chains and a ζ-containing homodimer or heterodimer. The disulfide-linked Ti α-β heterodimer is responsible for antigen recognition, but the short five amino acid cytoplasmic domains of Ti α and β are unlikely to be sufficient to couple to intracellular signaling pathways. In contrast, the structured features of the CD3 and ζ subunits suggest a role in signal transduction. Of these, the ζ chain, which is expressed as either a homodimer or heterodimer, has a short extracellular domain of only nine amino acids, but a larger 113 amino acid cytoplasmic domain. A tyrosine phosphoprotein, ZAP-70, has been identified that associates with ζ and undergoes tyrosine phosphorylation following TCR stimulation.Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZAP 70, ZAP-70
-
分子量
- approx. 70 kDa
-
基因ID
- 7535
-
NCBI登录号
- NP_001070
-
UniProt
- P43403
-
途径
- TCR Signaling, Ubiquitin Proteasome Pathway
抗原
-
