MIP 抗体
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北京 101111
Quick Overview for MIP 抗体 (ABIN498527)
抗原
See all MIP 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of AQP0 protein. (region surrounding Cys144)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity-Chromatography using epitope-specific immunogen
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anti-Major Intrinsic Protein of Lens Fiber (MIP) antibody
MIP 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Major Intrinsic Protein of Lens Fiber (MIP) (Internal Region) antibodyMIP 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Major Intrinsic Protein of Lens Fiber (MIP) (C-Term) antibodyMIP 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MIP (Major Intrinsic Protein of Lens Fiber (MIP))
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别名
- Aquaporin-0
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背景
- Aquaporins (AQPs) are a large family of integral membrane channel proteins that facilitate the transport of water through the cell membrane. Aquaporins are widely distributed and are involved in renal water absorption, generation of pulmonary secretions, lacrimation and the secretion and reabsorption of cerebrospinal fluid and aqueous humor. AQP0 is the most abundant endogenous protein in the plasma membrane of lens fiber cells where it functions not only as a water pore, but it is also involved in fiber-fiber adhesion and is crucial for fiber cell structure and organization. AQP0 contains an additional pore constriction, not seen in any other aquaporin structures, which may be responsible for pore gating. The closed AQP0 pore holds just three water molecules, which are spaced too far apart to form hydrogen bonds with each other. The C-terminal domain of AQP0 undergoes extensive post-translational modification, including many truncations, during lens aging due to the actions of m-Calpain, proteases or non-enzymatic mechanisms. These truncation sites may be involved in the development of cataracts.Synonyms: AQP0, Lens fiber major intrinsic protein, MIP, MIP26, MP26
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分子量
- approx. 28 kDa
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基因ID
- 4284
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NCBI登录号
- NP_036196
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UniProt
- P30301
抗原
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