E2F5 抗体
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北京 101111
Quick Overview for E2F5 抗体 (ABIN498519)
抗原
See all E2F5 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of E2F-5 protein. (region surrounding Leu142)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography using epitope-specific immunogen
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纯度
- > 95 % (by SDS-PAGE)
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anti-E2F5 (E2F5) (AA 66-93), (N-Term) antibody
E2F5 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB23726 unconjugated
anti-E2F5 (E2F5) (AA 101-200) antibodyE2F5 适用: 人, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-E2F5 (E2F5) antibodyE2F5 适用: 人 DB, ICC 宿主: 小鼠 Monoclonal 220D1a unconjugated
anti-E2F5 (E2F5) (AA 193-298) antibodyE2F5 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-E2F5 (E2F5) antibodyE2F5 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB8325 unconjugated
anti-E2F5 (E2F5) (AA 134-183) antibodyE2F5 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, 兔, 斑马鱼, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-E2F5 (E2F5) (AA 121-346) antibodyE2F5 适用: 人 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-E2F5 (E2F5) (N-Term) antibodyE2F5 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- E2F5
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别名
- E2F5
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背景
- The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5 and E2F-6, each of which forms heterodimers with a second protein, DP-1, forming an Synonyms: E2F transcription factor 5, E2F-5, Transcription factor E2F5, p130-binding
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分子量
- approx. 36 kDa
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基因ID
- 1875
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NCBI登录号
- NP_001077057
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UniProt
- Q15329
抗原
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