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- 抗原 See all SIAH2 抗体
- SIAH2 (Siah E3 Ubiquitin Protein Ligase 2 (SIAH2))
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SIAH2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- This antibody detects endogenous levels of Siah-2 protein. (region surrounding Glu266)
- 交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - 纯化方法
- Affinity chromatography
- 纯度
- > 95 % by SDS-PAGE
- Top Product
- Discover our top product SIAH2 Primary Antibody
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anti-Siah E3 Ubiquitin Protein Ligase 2 (SIAH2) (C-Term) antibody
SIAH2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Siah E3 Ubiquitin Protein Ligase 2 (SIAH2) (AA 1-72) antibodySIAH2 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Siah E3 Ubiquitin Protein Ligase 2 (SIAH2) (AA 1-100) antibodySIAH2 适用: 人, Pig, Drosophila melanogaster IHC, FACS, IF, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal 24E6H3 unconjugated
anti-Siah E3 Ubiquitin Protein Ligase 2 (SIAH2) (AA 216-244) antibodySIAH2 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB22563 unconjugated
anti-Siah E3 Ubiquitin Protein Ligase 2 (SIAH2) (C-Term) antibodySIAH2 适用: 人, 小鼠, 大鼠, 斑马鱼 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Siah E3 Ubiquitin Protein Ligase 2 (SIAH2) (C-Term) antibodySIAH2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Siah E3 Ubiquitin Protein Ligase 2 (SIAH2) (C-Term) antibodySIAH2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Siah E3 Ubiquitin Protein Ligase 2 (SIAH2) (N-Term) antibodySIAH2 适用: 人, 小鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Siah E3 Ubiquitin Protein Ligase 2 (SIAH2) (AA 215-264) antibodySIAH2 适用: 人, 小鼠, 大鼠, 斑马鱼, Pig, 犬, Cow, 豚鼠, 马, 兔, 猴, Bat, 小鸡, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Siah E3 Ubiquitin Protein Ligase 2 (SIAH2) (Middle Region) antibodySIAH2 适用: 人, 小鼠, 大鼠, 斑马鱼, 犬, Cow, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western Blot: 1/500 - 1/1000. Immunhistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1,0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- SIAH2 (Siah E3 Ubiquitin Protein Ligase 2 (SIAH2))
- 别名
- SIAH2 (SIAH2 产品)
- 别名
- hSiah2 antibody, Xsiah-2 antibody, siah2-A antibody, xsiah2 antibody, AA415433 antibody, Sinh2 antibody, siah E3 ubiquitin protein ligase 2 antibody, siah E3 ubiquitin protein ligase 2 L homeolog antibody, seven in absentia 2 antibody, SIAH2 antibody, siah2.L antibody, Siah2 antibody
- 背景
- SIAH-2 (seven in absentia homolog 2) is an E3 ligase that catalyzes ubiquitination and proteasome-mediated degradation of protein substrates. SIAH-2 encodes a 324 amino acid protein that shares 77 % identity with human SIAH-1 and 68 % identity with the Drosophila sina (seven in absentia) gene, on which development of the Drosophila R7 photoreceptor is dependent. SIAH-2 targets TRAF2 (which regulates cell responses to stress and cytokines through the regulation of key stress-signaling cascades) for degradation under stress conditions such as hypoxia. It targets HIF-1alpha prolyl hydroxylase 3 (PHD3) for degradation upon exposure to hypoxic conditions, which coincides with an increase in SIAH-2 transcription. SIAH-2 can decrease TNF-alpha-dependent induction of JNK activity and transcriptional activation of NFkappaB. SIAH-2 null mice subjected to hypoxia display an impaired respiratory response and reduced levels of hemoglobin.Synonyms: E3 ubiquitin-protein ligase SIAH2, Seven in absentia homolog 2, Siah-2
- 分子量
- approx. 38 kDa
- 基因ID
- 6478
- NCBI登录号
- NP_005058
- UniProt
- O43255
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