EIF4G2 抗体
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北京 101111
Quick Overview for EIF4G2 抗体 (ABIN498384)
抗原
See all EIF4G2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of DAP-5 protein. (region surrounding asn65)
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交叉反应 （详细）
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Species reactivity (expected):Mouse.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography
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纯度
- > 95 % by SDS-PAGE
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anti-Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2) (AA 750-850) antibody
EIF4G2 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2) (N-Term) antibodyEIF4G2 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2) (AA 811-889) antibodyEIF4G2 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3B5 unconjugated
anti-Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2) (C-Term) antibodyEIF4G2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2) (AA 766-815) antibodyEIF4G2 适用: 人, Cow, 兔, 犬, 马, 猴, Pig, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2) (N-Term) antibodyEIF4G2 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2) (AA 706-907) antibodyEIF4G2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2) (N-Term) antibodyEIF4G2 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2) (AA 811-889) antibodyEIF4G2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3E4 unconjugated
anti-Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2) (AA 125-175) antibodyEIF4G2 适用: 人 IHC, IF (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 1/500 - 1/1000. Immunohisochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- EIF4G2 (Eukaryotic Translation Initiation Factor 4 gamma 2 (EIF4G2))
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别名
- eIF4G2 / DAP5
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背景
- Death-associated protein 5 (DAP-5) (also known as p97 and NAT1) is a member of the eukaryotic translation initiation factor 4G (elF4G) family. DAP-5 is ubiquitously expressed and is highly conserved among species. In response to activated FAS or p53, caspase cleaves DAP-5 at position 790 to yield a C-terminal truncated protein which is capable of forming complexes with eIF4A and eIF3. DAP-5 has homology to the carboxy-terminal portion of eIF4G, but lacks the N-terminal region of eIF4G, which is responsible for association with the CAP binding protein eIF4E. By forming translationally inactive complexes with eIF4A and eIF3, but not with eIF4E, DAP-5 functions as a general repressor of translation. During apotosis, the caspase-activated DAP-5 can mediate CAP-independent translation at least from its own internal ribosome entry site, thus resulting in a positive feedback loop responsible for the continuous translation of DAP-5. DAP-5 is also required for cellular differentiation, as it controls specific gene expression pathways.Synonyms: AAG1, Death-associated protein 5, Eukaryotic translation initiation factor 4 gamma 2, FLJ41344, NAT1, eIF-4-gamma 2, p97
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分子量
- approx. 90 kDa
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基因ID
- 1982
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NCBI登录号
- NP_001409
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UniProt
- P78344
抗原
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