GTF3C3 抗体
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北京 101111
Quick Overview for GTF3C3 抗体 (ABIN498362)
抗原
See all GTF3C3 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of TFIIIC102 protein. (region surrounding Glu131)
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交叉反应 （详细）
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Species reactivity (expected):Mouse.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography
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纯度
- > 95 % by SDS-PAGE
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anti-General Transcription Factor IIIC, Polypeptide 3, 102kDa (GTF3C3) antibody
GTF3C3 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-General Transcription Factor IIIC, Polypeptide 3, 102kDa (GTF3C3) antibodyGTF3C3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-General Transcription Factor IIIC, Polypeptide 3, 102kDa (GTF3C3) antibodyGTF3C3 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-General Transcription Factor IIIC, Polypeptide 3, 102kDa (GTF3C3) (AA 112-214) antibodyGTF3C3 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 3D9 unconjugated
anti-General Transcription Factor IIIC, Polypeptide 3, 102kDa (GTF3C3) (N-Term) antibodyGTF3C3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-General Transcription Factor IIIC, Polypeptide 3, 102kDa (GTF3C3) antibodyGTF3C3 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-General Transcription Factor IIIC, Polypeptide 3, 102kDa (GTF3C3) (N-Term) antibodyGTF3C3 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-General Transcription Factor IIIC, Polypeptide 3, 102kDa (GTF3C3) (AA 1-886) antibodyGTF3C3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-General Transcription Factor IIIC, Polypeptide 3, 102kDa (GTF3C3) (AA 112-214) antibodyGTF3C3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-General Transcription Factor IIIC, Polypeptide 3, 102kDa (GTF3C3) (AA 101-150) antibodyGTF3C3 适用: 人, 小鼠 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- GTF3C3 (General Transcription Factor IIIC, Polypeptide 3, 102kDa (GTF3C3))
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别名
- GTF3C3
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背景
- RNA polymerase (pol) III synthesizes tRNA, 5s rRNA, 7SL RNA and U6 snRNA and is overexpressed in many transformed cell lines and tumors in vivo, since cells must duplicate its protein components before division. Therefore, in order to maintain rapid growth, cells must produce a high level of Pol III transcribed RNA, which requires the presence of the TFIIIB and TFIIIC2 transcription factor complexes. The TFIIIC2 complex is composed of five subunits, TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, that are overexpressed in adenovirus transformed cells as well as in malignant cells in vivo, such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be a key component in the deregulation of malignant cells. The TFIIIB complex includes the TATA-binding protein (TBP), TFIIB-related factor 1 (BRF1) and TFIIIB, the expression of which are also upregulated in transformed cells. In many carcinomas, the tumor suppressors retinoblastoma (RB) and p53 are inactivated, which affects their ability to bind and inactivate the function of TFIIIB.Synonyms: General transcription factor 3C polypeptide 3, TFIIIC 102 kDa subunit, TFIIIC gamma, TFIIIC102, Transcription factor IIIC subunit gamma
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分子量
- approx. 101 kDa
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基因ID
- 9330
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NCBI登录号
- NP_036218
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UniProt
- Q9Y5Q9
抗原
-
