DBF4 抗体
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Quick Overview for DBF4 抗体 (ABIN498361)
抗原
See all DBF4 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of Dbf4 protein. (region surrounding Ile18)
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交叉反应 （详细）
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Species reactivity (expected):Mouse.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography
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纯度
- > 95 % by SDS-PAGE
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anti-DBF4 Homolog (DBF4) (AA 2-98) antibody
DBF4 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 6G9 unconjugated
anti-DBF4 Homolog (DBF4) (AA 2-98) antibodyDBF4 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) (Internal Region) antibodyDBF4 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) (AA 101-281) antibodyDBF4 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) antibodyDBF4 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB3815 unconjugated Recombinant Antibody
anti-DBF4 Homolog (DBF4) antibodyDBF4 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB5370 unconjugated Recombinant Antibody
anti-DBF4 Homolog (DBF4) (AA 295-665) antibodyDBF4 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) (N-Term) antibodyDBF4 适用: 人, Cow, Pig WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) (N-Term) antibodyDBF4 适用: 人, 小鼠, 猴 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-DBF4 Homolog (DBF4) (AA 1-674) antibodyDBF4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- DBF4 (DBF4 Homolog (DBF4))
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别名
- DBF4 / ASK
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背景
- The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase alpha-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosome separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.Synonyms: Activator of S phase kinase, Chiffon homolog A, DBF4-type zinc finger-containing protein 1, DBF4A, Protein DBF4 homolog A, ZDBF1
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分子量
- approx. 77 kDa
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基因ID
- 10926
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NCBI登录号
- NP_006707
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UniProt
- Q9UBU7
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途径
- Mitotic G1-G1/S Phases, DNA Replication
抗原
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