电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

DBF4 抗体

DBF4 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN498361
发货至: 中国
  • 抗原 See all DBF4 抗体
    DBF4 (DBF4 Homolog (DBF4))
    适用
    • 45
    • 16
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    人, 小鼠
    宿主
    • 41
    • 4
    • 1
    克隆类型
    • 41
    • 5
    多克隆
    标记
    • 21
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This DBF4 antibody is un-conjugated
    应用范围
    • 35
    • 16
    • 13
    • 13
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    特异性
    This antibody detects endogenous levels of Dbf4 protein. (region surrounding Ile18)
    交叉反应 (详细)
    Species reactivity (expected):Mouse.
    Species reactivity (tested):Human.
    纯化方法
    Affinity chromatography
    纯度
    > 95 % by SDS-PAGE
    Top Product
    Discover our top product DBF4 Primary Antibody
  • 应用备注
    Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1,0 mg/mL
    缓冲液
    Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原
    DBF4 (DBF4 Homolog (DBF4))
    别名
    DBF4 / ASK (DBF4 产品)
    别名
    ASK antibody, CHIF antibody, DBF4A antibody, ZDBF1 antibody, RGD1305854 antibody, DBF4 antibody, LOC100229596 antibody, AA545217 antibody, Ask antibody, dbf4a antibody, xdbf4 antibody, ChDBF4 antibody, DBF4 zinc finger antibody, DBF4 zinc finger L homeolog antibody, DBF4 antibody, dbf4 antibody, Dbf4 antibody, dbf4.L antibody
    背景
    The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase alpha-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosome separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.Synonyms: Activator of S phase kinase, Chiffon homolog A, DBF4-type zinc finger-containing protein 1, DBF4A, Protein DBF4 homolog A, ZDBF1
    分子量
    approx. 77 kDa
    基因ID
    10926
    NCBI登录号
    NP_006707
    UniProt
    Q9UBU7
    途径
    Mitotic G1-G1/S Phases, DNA Replication
You are here:
客服