TSG101 抗体
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北京 101111
Quick Overview for TSG101 抗体 (ABIN498353)
抗原
See all TSG101 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of Tsg 101 protein. (region surrounding Glu303)
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交叉反应 (详细)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography
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纯度
- > 95 % by SDS-PAGE
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应用备注
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- TSG101 (Tumor Susceptibility Gene 101 (TSG101))
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别名
- TSG101
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背景
- It is now well accepted that the transformation of a normal cell to one that is malignant can result from mutations in genes that encode proteins with key regulatory functions. Examples include the retinoblastoma gene product (Rb p110), p53, VHL and APC. Using a novel cloning strategy that allows the isolation of previously uncharacterized genes encoding selectable recessive phenotypes, an additional tumor suppressor gene has been identified. This gene, termed tsg 101 for tumor susceptibility gene 101, encodes a stathmin binding domain protein. When expression of this growth inhibitory gene is blocked in NIH/3T3 cells using antisense mRNA, the cells exhibit a transformed phenotype and are tumorigenic in SL6 mice.Synonyms: ESCRT-I complex subunit TSG101, Tumor susceptibility gene 101 protein
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分子量
- approx. 45 kDa
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基因ID
- 7251
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NCBI登录号
- NP_006283
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UniProt
- Q99816
抗原
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