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TSG101 抗体

The 兔 多克隆 anti-TSG101 antibody is suitable to detect TSG101 in samples from 人, 小鼠 和 大鼠. It has been validated for WB, IF 和 IHC (p).
产品编号 ABIN498353
发货至: 中国
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Quick Overview for TSG101 抗体 (ABIN498353)

抗原

See all TSG101 抗体
TSG101 (Tumor Susceptibility Gene 101 (TSG101))

适用

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人, 小鼠, 大鼠

宿主

  • 77
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克隆类型

  • 56
  • 41
多克隆

标记

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This TSG101 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 特异性

    This antibody detects endogenous levels of Tsg 101 protein. (region surrounding Glu303)

    交叉反应 (详细)

    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.

    纯化方法

    Affinity chromatography

    纯度

    > 95 % by SDS-PAGE
  • 应用备注

    Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1,0 mg/mL

    缓冲液

    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    TSG101 (Tumor Susceptibility Gene 101 (TSG101))

    别名

    TSG101

    背景

    It is now well accepted that the transformation of a normal cell to one that is malignant can result from mutations in genes that encode proteins with key regulatory functions. Examples include the retinoblastoma gene product (Rb p110), p53, VHL and APC. Using a novel cloning strategy that allows the isolation of previously uncharacterized genes encoding selectable recessive phenotypes, an additional tumor suppressor gene has been identified. This gene, termed tsg 101 for tumor susceptibility gene 101, encodes a stathmin binding domain protein. When expression of this growth inhibitory gene is blocked in NIH/3T3 cells using antisense mRNA, the cells exhibit a transformed phenotype and are tumorigenic in SL6 mice.Synonyms: ESCRT-I complex subunit TSG101, Tumor susceptibility gene 101 protein

    分子量

    approx. 45 kDa

    基因ID

    7251

    NCBI登录号

    NP_006283

    UniProt

    Q99816
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