DGKE 抗体
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北京 101111
Quick Overview for DGKE 抗体 (ABIN498319)
抗原
See all DGKE 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of DGK-ε protein. (region surrounding Ser194)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography using epitope-specific immunogen
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纯度
- > 95 % (by SDS-PAGE)
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anti-Diacylglycerol Kinase, epsilon 64kDa (DGKE) (AA 141-240) antibody
DGKE 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 7E1 unconjugated
anti-Diacylglycerol Kinase, epsilon 64kDa (DGKE) (AA 40-260) antibodyDGKE 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Diacylglycerol Kinase, epsilon 64kDa (DGKE) (Internal Region) antibodyDGKE 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Diacylglycerol Kinase, epsilon 64kDa (DGKE) (AA 243-502) antibodyDGKE 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Diacylglycerol Kinase, epsilon 64kDa (DGKE) (AA 1-100) antibodyDGKE 适用: 小鼠 WB, ELISA, IF (cc), IF (p), ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Diacylglycerol Kinase, epsilon 64kDa (DGKE) (N-Term) antibodyDGKE 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Diacylglycerol Kinase, epsilon 64kDa (DGKE) (N-Term) antibodyDGKE 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Diacylglycerol Kinase, epsilon 64kDa (DGKE) (N-Term) antibodyDGKE 适用: 人, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- DGKE (Diacylglycerol Kinase, epsilon 64kDa (DGKE))
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别名
- DAG Kinase epsilon
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背景
- Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-α (80 kDa), -β (90 kDa), and -γ (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK-δ (130 kDa) and DGK-?(130 kDa) contain N-terminal pleckstrin homology (PH) domains and are classified as type II. Human DGK-ε (64 kDa) contains no identifiable regulatory domains and is classified as a type III DGK. Human DGK-ζ (104 kDa) and -iota (130 kDa) possess C-terminal ankyrin repeats and are classified as type IV DGKs. Human DGK-θ (110 kDa) contains 3 cysteine-rich domains and a PH domain and is classified as a type V DGK.Synonyms: DAGK5, DGK-epsilon, DGKE, Diacylglycerol kinase epsilon, Diglyceride kinase epsilon
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分子量
- approx. 60 kDa
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基因ID
- 8526
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NCBI登录号
- NP_003638
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UniProt
- P52429
抗原
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