Aconitase 1 抗体
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Quick Overview for Aconitase 1 抗体 (ABIN498149)
抗原
See all Aconitase 1 (ACO1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of IRP-1 protein.
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity Chromatography using epitope-specific immunogen.
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anti-Aconitase 1 (ACO1) (AA 329-443) antibody
ACO1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Aconitase 1 (ACO1) (AA 251-443) antibodyACO1 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro), FACS 宿主: 兔 Polyclonal unconjugated
anti-Aconitase 1 (ACO1) (N-Term) antibodyACO1 适用: 人, 小鼠, 大鼠, 兔, Cow, 犬, 豚鼠, 马, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Aconitase 1 (ACO1) (AA 26-243) antibodyACO1 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro), FACS 宿主: 兔 Polyclonal unconjugated
anti-Aconitase 1 (ACO1) (AA 780-889) antibodyACO1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 2C1 unconjugated
anti-Aconitase 1 (ACO1) (AA 1-889) antibodyACO1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Aconitase 1 (ACO1) (AA 508-745) antibodyACO1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Aconitase 1 (ACO1) (AA 527-719) antibodyACO1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Aconitase 1 (ACO1) (N-Term) antibodyACO1 适用: 人, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Aconitase 1 (ACO1) (N-Term) antibodyACO1 适用: 人, 大鼠, Cow WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot: 1/500-1/1000. Immunoflourescence: 1/50-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Aconitase 1 (ACO1)
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别名
- ACO1 / IREB1
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背景
- The insulin receptor substrate-1 (IRS-1), a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor. The association of 14-3-3 with IRS-1 increases significantly upon treatment with okadaic acid, a potent serine/ threonine phosphatase inhibitor. Therefore, the association of 14-3-3 protein may play a role in the regulation of insulin sensitivity by interrupting the association between the insulin receptor and IRS-1.Synonyms: Aconitase, Citrate hydro-lyase, Cytoplasmic aconitate hydratase, Ferritin repressor protein, IRP1, Iron regulatory protein 1, Iron-responsive element-binding protein 1
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分子量
- approx. 98 kDa
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基因ID
- 48
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NCBI登录号
- NP_002188
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UniProt
- P21399
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途径
- Transition Metal Ion Homeostasis
抗原
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