PLA2G4A 抗体 (Internal Region)
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Quick Overview for PLA2G4A 抗体 (Internal Region) (ABIN498079)
抗原
See all PLA2G4A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- C-QEKTFRQQRK EHIR
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交叉反应 （详细）
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Species reactivity (expected):Rat.
Species reactivity (tested):Human, Mouse. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide from the internal region of the protein sequence according to NP_077734.1. Genename: PLA2G4A
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anti-Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) (AA 563-749) antibody
PLA2G4A 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) (N-Term) antibodyPLA2G4A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) (AA 1-178) antibodyPLA2G4A 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) (AA 1-178) antibodyPLA2G4A 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C19 unconjugated
anti-Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) (pSer505) antibodyPLA2G4A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) (AA 513-541) antibodyPLA2G4A 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB21320 unconjugated
anti-Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) (Internal Region) antibodyVerified PLA2G4A 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) (AA 1-110) antibodyPLA2G4A 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 10G5 unconjugated
anti-Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) (AA 379-533) antibodyPLA2G4A 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A) (AA 471-520) antibodyPLA2G4A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Detection Limit: 1/32000. Western blot: 1-3 μg/mL. Detects a band of Approx 90 kDa in lysates of cell lines HeLa and NIH3T3.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Human, Mouse. Expected from sequence similarity: Rat. Add. Information: Calculated Molecular Weight: 85.0 kDa according to NP_077734.1 -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))
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别名
- PLA2G4A
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背景
- Cytosolic phospholipase A2 (cPLA2) is a ubiquitously distributed enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and release arachidonic acid. cPLA2 has been implicated in diverse cellular responses such as mitogenesis, differentiation, inflammation and cytotoxicity. Calcium binding to the amino-terminal CalB domain of cPLA2 promotes the translocation of cPLA2 from cytosol to membrane, where cPLA2 cleaves arachidonic acid from natural membrane.Synonyms: Cytosolic phospholipase A2, Phospholipase A2 group IVA, cPLA2, phospholipase A2
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基因ID
- 5321
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NCBI登录号
- NP_077734
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UniProt
- P47712
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途径
- Inositol Metabolic Process, G-protein mediated Events, VEGF Signaling
抗原
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