Peptide ELISA: Detection Limit: 1/32000. Western blot: 1-3 μg/mL. Detects a band of Approx 90 kDa in lysates of cell lines HeLa and NIH3T3. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Human, Mouse. Expected from sequence similarity: Rat. Add. Information: Calculated Molecular Weight: 85.0 kDa according to NP_077734.1
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原
PLA2G4A
(Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))
Cytosolic phospholipase A2 (cPLA2) is a ubiquitously distributed enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and release arachidonic acid. cPLA2 has been implicated in diverse cellular responses such as mitogenesis, differentiation, inflammation and cytotoxicity. Calcium binding to the amino-terminal CalB domain of cPLA2 promotes the translocation of cPLA2 from cytosol to membrane, where cPLA2 cleaves arachidonic acid from natural membrane.Synonyms: Cytosolic phospholipase A2, Phospholipase A2 group IVA, cPLA2, phospholipase A2