Dystroglycan 抗体 (Internal Region)
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Quick Overview for Dystroglycan 抗体 (Internal Region) (ABIN498078)
抗原
See all Dystroglycan (DAG1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- C-HVGKHEYFMH ATDK
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特异性
- This antibody is expected to recognize both the precursor and the mature Alpha-Dystroglycan, but not the mature Beta-Dystroglycan.
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交叉反应 (详细)
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Species reactivity (expected):Canine (Dog), Bovine.
Species reactivity (tested):Human, Mouse, Rat. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide from the internal region of the protein sequence according to NP_004384.3. Genename: DAG1
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应用备注
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Peptide ELISA: Detection Limit: 1/128000. Western blot: 0.2-0.6 μg/mL. Detects a band of Approx 100 kDa in Human Skeletal Muscle lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))
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别名
- Dystroglycan
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背景
- Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha Dystroglycan and a 43 kDa transmembrane protein, beta Dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin.Synonyms: DAG1, Dystrophin-associated glycoprotein 1
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基因ID
- 1605
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NCBI登录号
- NP_004384
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UniProt
- Q14118
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途径
- Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus
抗原
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