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Dystroglycan 抗体 (Internal Region)

The 山羊 多克隆 anti-Dystroglycan antibody (ABIN498078) specifically detects Dystroglycan in WB 和 EIA. The antibody is reactive with 人, 小鼠, 大鼠, Cow 和 犬 samples.
产品编号 ABIN498078
发货至: 中国
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Quick Overview for Dystroglycan 抗体 (Internal Region) (ABIN498078)

抗原

See all Dystroglycan (DAG1) 抗体
Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

适用

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人, 小鼠, 大鼠, Cow, 犬

宿主

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山羊

克隆类型

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多克隆

标记

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This Dystroglycan antibody is un-conjugated

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 抗原表位

    • 15
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    Internal Region

    序列

    C-HVGKHEYFMH ATDK

    特异性

    This antibody is expected to recognize both the precursor and the mature Alpha-Dystroglycan, but not the mature Beta-Dystroglycan.

    交叉反应 (详细)

    Species reactivity (expected):Canine (Dog), Bovine.
    Species reactivity (tested):Human, Mouse, Rat.

    纯化方法

    Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

    免疫原

    Peptide from the internal region of the protein sequence according to NP_004384.3. Genename: DAG1
  • 应用备注

    Peptide ELISA: Detection Limit: 1/128000. Western blot: 0.2-0.6 μg/mL. Detects a band of Approx 100 kDa in Human Skeletal Muscle lysates.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

    别名

    Dystroglycan

    背景

    Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha Dystroglycan and a 43 kDa transmembrane protein, beta Dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin.Synonyms: DAG1, Dystrophin-associated glycoprotein 1

    基因ID

    1605

    NCBI登录号

    NP_004384

    UniProt

    Q14118

    途径

    Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus
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