POU3F3 抗体 (Internal Region)
Quick Overview for POU3F3 抗体 (Internal Region) (ABIN498034)
抗原
See all POU3F3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- C-HMLSHAHQWV TAL
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特异性
- Recognizes POU3F3 / BRN1 / OCT8.
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交叉反应 （详细）
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Species reactivity (expected):Human.
Species reactivity (tested):Mouse, Rat. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence from the internal region of the protein sequence according to NP_006227.1. Genename: POU3F3
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anti-POU Class 3 Homeobox 3 (POU3F3) (Internal Region) antibody
Verified POU3F3 适用: 小鼠 WB, ELISA, FACS, IF 宿主: 山羊 Polyclonal unconjugated
anti-POU Class 3 Homeobox 3 (POU3F3) (N-Term) antibodyPOU3F3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-POU Class 3 Homeobox 3 (POU3F3) (AA 381-407), (C-Term) antibodyPOU3F3 适用: 人, 斑马鱼 WB 宿主: 兔 Polyclonal RB44742 unconjugated
anti-POU Class 3 Homeobox 3 (POU3F3) (C-Term) antibodyPOU3F3 适用: 人, 小鼠, 大鼠, 犬, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-POU Class 3 Homeobox 3 (POU3F3) (AA 401-500) antibodyPOU3F3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-POU Class 3 Homeobox 3 (POU3F3) (AA 35-84) antibodyPOU3F3 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-POU Class 3 Homeobox 3 (POU3F3) (AA 381-407) antibodyPOU3F3 适用: 斑马鱼 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Limit Dilution: 1/4000. Western blot: 1-3 μg/mL. Approx 48 kDa band observed in Mouse and Rat Spinal Cordlysates (calculated MW of 50.8 kDa according to Human NP_006227.1 and 50.8 kDaaccording to Mouse NP_032926.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- POU3F3 (POU Class 3 Homeobox 3 (POU3F3))
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别名
- POU3F3
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背景
- POU3F3, also known as Brn1, is a member of the POU family of transcription factors that is predominantly expressed in the brain. It contains a characteristic POU domain and a homeobox DNA-binding domain. POU3F3 plays a role in the development of the brain and kidney by upregulating transcription of key genes in these tissues. For example, POU3F3 interacts with the proneural protein Mash1 and POU3F2 (Brn2) on the promoter of the Notch ligand Delta1 to activate Delta1 transcription, a key step in neurogenesis.Synonyms: BRN1, Brain-1, Brain-specific homeobox/POU domain protein 1, Brn-1, OTF-8, OTF8, Oct-8, Octamer-binding protein 8, Octamer-binding transcription factor 8, POU domain class 3 transcription factor 3
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基因ID
- 5455
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NCBI登录号
- NP_006227
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UniProt
- P20264
抗原
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